Corticosteroid-dependent sodium transport in a novel immortalized mouse collecting duct principal cell line

Détails

ID Serval
serval:BIB_BE139A17717B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Corticosteroid-dependent sodium transport in a novel immortalized mouse collecting duct principal cell line
Périodique
Journal of the American Society of Nephrology
Auteur(s)
Bens  M., Vallet  V., Cluzeaud  F., Pascual-Letallec  L., Kahn  A., Rafestin-Oblin  M. E., Rossier  B. C., Vandewalle  A.
ISSN
1046-6673
Statut éditorial
Publié
Date de publication
05/1999
Peer-reviewed
Oui
Volume
10
Numéro
5
Pages
923-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
The final control of sodium balance takes place in the cortical collecting duct (CCD) of the nephron, where corticosteroid hormones regulate sodium reabsorption by acting through mineralocorticoid (MR) and/or glucocorticoid (GR) receptors. A clone of principal CCD cells (mpkCCDc14) has been established that is derived from a transgenic mouse (SV40 large T antigen under the control of the SV40 enhancer/L-type pyruvate kinase promoter). Cells grown on filters form polarized monolayers with high electrical transepithelial resistance (R(T) approximately 4700 ohm x cm2) and potential difference (P(D) approximately -50 mV) and have an amiloride-sensitive electrogenic sodium transport, as assessed by the short-circuit current method (Isc approximately 11 microA/cm2). Reverse transcription-PCR experiments using rat MR primers, [3H]aldosterone, and [3H]dexamethasone binding and competition studies indicated that the mpkCCDc14 cells exhibit specific MR and GR. Aldosterone increased Isc in a dose- (10(-10) to 10(-6) M) and time-dependent (2 to 72 h) manner, whereas corticosterone only transiently increased Isc (2 to 6 h). Consistent with the expression of 11beta-hydroxysteroid dehydrogenase type 2, which metabolizes glucocorticoids to inactive 11-dehydroderivates, carbenoxolone potentiated the corticosterone-stimulated Isc. Aldosterone (5x10(-7) M)-induced Isc (fourfold) was associated with a three- to fivefold increase in alpha-ENaC mRNA (but not in those for beta- or gamma-ENaC) and three- to 10-fold increases in alpha-ENaC protein synthesis. In conclusion, this new immortalized mammalian CCD clonal cell line has retained a high level of epithelial differentiation and sodium transport stimulated by aldosterone and therefore represents a useful mammalian cell system for identifying the genes controlled by aldosterone.
Mots-clé
Adenosine/pharmacology Adrenal Cortex Hormones/*pharmacology Amiloride/pharmacology Animals Biological Transport/drug effects Carbenoxolone/pharmacology Cell Line, Transformed Cells, Cultured Corticosterone/pharmacology Dose-Response Relationship, Drug Electrophysiology Epithelial Sodium Channel Kidney Tubules, Collecting/cytology/*metabolism/physiology Male Mice Receptors, Glucocorticoid/physiology Receptors, Mineralocorticoid/physiology Sodium/*metabolism Sodium Channels/physiology Substrate Specificity Time Factors
Pubmed
Web of science
Création de la notice
24/01/2008 13:01
Dernière modification de la notice
20/08/2019 15:32
Données d'usage