Expression of early and late activation markers on peripheral blood T lymphocytes does not reliably reflect immune events in transplanted hearts

Détails

ID Serval
serval:BIB_BDF2AFC25AAF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of early and late activation markers on peripheral blood T lymphocytes does not reliably reflect immune events in transplanted hearts
Périodique
Clin Transplant
Auteur(s)
Loertscher R., Forbes R. D., Halabi G., Lavery P., Quinn T.
ISSN
0902-0063 (Print)
ISSN-L
0902-0063
Statut éditorial
Publié
Date de publication
06/1994
Peer-reviewed
Oui
Volume
8
Numéro
3 Pt 1
Pages
230-8
Langue
anglais
Notes
Loertscher, R
Forbes, R D
Halabi, G
Lavery, P
Quinn, T
eng
Comparative Study
Research Support, Non-U.S. Gov't
Denmark
Clin Transplant. 1994 Jun;8(3 Pt 1):230-8.
Résumé
Monoclonal antibodies directed against early (receptors for interleukin-2 and transferrin [IL-2R, TfR]) and late (PTA1, alpha 1 integrin VLA-1) activation antigens were used as probes to monitor cardiac transplant patients for episodes of acute graft rejection. Age- and sex-matched patient control groups consisting of 11 patients awaiting cardiac transplantation and 13 kidney transplant recipients with long-term grafts, respectively, were used to define an upper limit for normal activation antigen expression (mean + 3 SD) in patients. Expression of all cell markers was significantly higher in both patient control groups than in healthy control individuals. Therefore, the level of activation marker expression in heart patients awaiting transplantation was used as comparison for the patient population under study. Sequential monitoring of 24 heart transplant recipients failed to demonstrate a significant correlation of increased activation marker expression with clinical events of immune activation. Subsequently 62 consecutive endomyocardial biopsy scores in 36 patients were compared with the expression of IL-2R, TfR and VLA-1 on peripheral blood T cells. Neither increased cellular infiltration of the endocardium, nor of the myocardium, was associated with increasing proportions of IL-2R, TfR, or VLA-1 positive T cells. Elevated T-cell expression of the three markers combined indicated acute graft rejection with a sensitivity, specificity, and overall accuracy of 38%, 52%, and 43%, respectively. Acute graft rejection in biopsies with associated myofiber damage (biopsy rejection scores 2 and 3A,B) was not associated with a change in the proportion of activated T cells in circulation within the first 6 months after transplantation.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Adult, *Antigens, CD, Antigens, CD27/genetics/*immunology, Antigens, Differentiation, T-Lymphocyte/genetics/immunology, Biopsy, Blood, Female, Forecasting, Gene Expression, Graft Rejection/immunology, Heart Transplantation/*immunology/pathology, Humans, Integrins/genetics/immunology, Lectins, C-Type, Longitudinal Studies, Lymphocyte Activation/*immunology, Male, Middle Aged, Myocardium/pathology, Receptors, Interleukin-2/genetics/immunology, Receptors, Transferrin/genetics/immunology, Receptors, Very Late Antigen/genetics/immunology, T-Lymphocytes/*immunology
Pubmed
Création de la notice
01/11/2017 12:46
Dernière modification de la notice
20/08/2019 16:32
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