Liquid chromatography/mass spectrometry in anabolic steroid analysis--optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization.

Détails

ID Serval
serval:BIB_BD97A76032BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Liquid chromatography/mass spectrometry in anabolic steroid analysis--optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization.
Périodique
Journal of mass spectrometry : JMS
Auteur(s)
Leinonen A., Kuuranne T., Kostiainen R.
ISSN
1076-5174 (Print)
ISSN-L
1076-5174
Statut éditorial
Publié
Date de publication
07/2002
Peer-reviewed
Oui
Volume
37
Numéro
7
Pages
693-698
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The applicability of liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the detection of the free anabolic steroid fraction in human urine was examined. Electrospray ionization (ESI), atmospheric pressure chemical ionization and atmospheric pressure photoionization methods were optimized regarding eluent composition, ion source parameters and fragmentation. The methods were compared with respect to specificity and detection limit. Although all methods proved suitable, LC/ESI-MS/MS with a methanol-water gradient including 5 mM ammonium acetate and 0.01% acetic acid was found best for the purpose. Multiple reaction monitoring allowed the determination of steroids in urine at low nanogram per milliliter levels. LC/MS/MS exhibited high sensitivity and specificity for the detection of free steroids and may be a suitable technique for screening for the abuse of anabolic steroids in sports.

Mots-clé
Anabolic Agents/urine, Chromatography, Liquid, Doping in Sports, Humans, Indicators and Reagents, Photochemistry, Spectrometry, Mass, Electrospray Ionization
Pubmed
Web of science
Création de la notice
02/05/2017 13:37
Dernière modification de la notice
20/08/2019 15:31
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