From Cultured Rodent Neurons to Human Brain Tissue: Model Systems for Pharmacological and Translational Neuroscience.

Détails

Ressource 1Télécharger: MS-Wellbourne-Wood-Chatton-Model systems_SERVAL.pdf (1749.95 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_BD6E6A8EABFC
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
From Cultured Rodent Neurons to Human Brain Tissue: Model Systems for Pharmacological and Translational Neuroscience.
Périodique
ACS chemical neuroscience
Auteur(s)
Wellbourne-Wood J., Chatton J.Y.
ISSN
1948-7193 (Electronic)
ISSN-L
1948-7193
Statut éditorial
Publié
Date de publication
15/08/2018
Peer-reviewed
Oui
Volume
9
Numéro
8
Pages
1975-1985
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Résumé
To investigate the enormous complexity of the functional and pathological brain there are a number of possible experimental model systems to choose from. Depending on the research question choosing the appropriate model may not be a trivial task, and given the dynamic and intricate nature of an intact living brain several models might be needed to properly address certain questions. In this review, we aim to provide an overview of neural cell and tissue culture, reflecting on historic methodological milestones and providing a brief overview of the state-of-the-art. We additionally present an example of an effective model system pipeline, composed of dissociated mouse cultures, organotypics, acute mouse brain slices, and acute human brain slices, in that order. The sequential use of these four model systems allows a balance and progression from experimental control to human applicability, and provides a meta-model that can help validate basic research findings in a translational setting. We then conclude with a few remarks regarding the necessity of an integrated approach when performing translational and neuropharmacological studies.
Mots-clé
Animals, Brain/drug effects, Brain/physiology, Cells, Cultured, Humans, Models, Biological, Neurons/drug effects, Neurons/physiology, Organoids/drug effects, Organoids/physiology, Tissue Culture Techniques, Translational Medical Research, Tissue culture, astrocytes, brain slices, model systems, neurons, primary cell culture
Pubmed
Web of science
Création de la notice
06/06/2018 13:01
Dernière modification de la notice
09/10/2019 5:09
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