Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line.

Détails

ID Serval
serval:BIB_BD4507467DBB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line.
Périodique
In Vitro Cellular and Developmental Biology. Animal
Auteur(s)
Blum W., Pecze L., Felley-Bosco E., Worthmüller-Rodriguez J., Wu L., Vrugt B., de Perrot M., Schwaller B.
ISSN
1543-706X (Electronic)
ISSN-L
1071-2690
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
51
Numéro
7
Pages
714-721
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Mesothelial cells are susceptible to asbestos fiber-induced cytotoxicity and on longer time scales to transformation; the resulting mesothelioma is a highly aggressive neoplasm that is considered as incurable at the present time Zucali et al. (Cancer Treatment Reviews 37:543-558, 2011). Only few murine cell culture models of immortalized mesothelial cells and mesothelioma cell lines exist to date. We generated SV40-immortalized cell lines derived from wild-type (WT) and neurofibromatosis 2 (merlin) heterozygote (Nf2+/-) mice, both on a commonly used genetic background, C57Bl/6J. All immortalized mesothelial clones consistently grow in DMEM supplemented with fetal bovine serum. Cells can be passaged for more than 40 times without any signs of morphological changes or a decrease in proliferation rate. The tumor suppressor gene NF2 is one of the most frequently mutated genes in human mesothelioma, but its detailed function is still unknown. Thus, these genotypically distinct cell lines likely relevant for malignant mesothelioma formation are expected to serve as useful in vitro models, in particular to compare with in vivo studies in mice of the same genotype. Furthermore, we generated a novel murine mesothelioma cell line RN5 originating from an Nf2+/- mouse subjected to repeated crocidolite exposure. RN5 cells are highly tumorigenic.
Pubmed
Open Access
Oui
Création de la notice
12/08/2015 7:43
Dernière modification de la notice
20/08/2019 15:31
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