Methodological considerations when conducting in vitro, air-liquid interface exposures to engineered nanoparticle aerosols

Détails

ID Serval
serval:BIB_BD2D7CC70CB0
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Methodological considerations when conducting in vitro, air-liquid interface exposures to engineered nanoparticle aerosols
Périodique
Critical Reviews in Toxicology
Auteur(s)
Secondo Lynn E., Liu Nathan J., Lewinski Nastassja A.
ISSN
1547-6898 (Electronic)
ISSN-L
1040-8444
Statut éditorial
Publié
Date de publication
03/2017
Peer-reviewed
Oui
Volume
47
Numéro
3
Pages
225-262
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Little consistency exists in the methodology for toxicological testing of aerosolized nanoparticles used in in vitro, air-interfaced culture (AIC) exposure systems for engineered nanoparticles (ENPs) risk-assessment, preventing inter-laboratory comparisons to identify dose thresholds for adverse effects. These inconsistencies result from heterogeneity in particle types, exposure durations, exposure systems, and dose metrics reported. We screened 10,241 studies in the literature for toxicological assessment of ENPs, resulting in 110 publications included after meeting eligibility criteria. In this review, we critically analyzed methodology within these studies to answer whether: (1) the administered dose or the deposited dose correlated better with biological response, (2) a difference existed between various AIC exposure systems when depositing the same dose, (3) consistent results were generated for nanomaterials with similar physico-chemical properties, (4) the deposited dose in vitro correlated to the deposited dose in vivo, and (5) AIC studies reliably modeled acute toxicity in vivo. Methods used in delivering, measuring, and reporting ENP aerosol doses in vitro are summarized. Dosimetry and biological response comparisons of AIC, conventional suspensions, and in vivo exposures are discussed through case studies on silver, zinc oxide, titanium dioxide, and multi-walled carbon nanotube exposures. Finally, based on these findings, recommendations are offered for design of future AIC experiments to aid standardization and comparisons of results.

Mots-clé
Nanoparticles, Inhalation Exposure, Culture Techniques
Pubmed
Web of science
Création de la notice
24/09/2016 10:39
Dernière modification de la notice
20/08/2019 15:31
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