Concordance among digital gene expression, microarrays, and qPCR when measuring differential expression of microRNAs.

Détails

ID Serval
serval:BIB_BCC9074E34BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Concordance among digital gene expression, microarrays, and qPCR when measuring differential expression of microRNAs.
Périodique
Biotechniques
Auteur⸱e⸱s
Pradervand S., Weber J., Lemoine F., Consales F., Paillusson A., Dupasquier M., Thomas J., Richter H., Kaessmann H., Beaudoing E., Hagenbüchle O., Harshman K.
ISSN
1940-9818[electronic], 0736-6205[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
48
Numéro
3
Pages
219-222
Langue
anglais
Résumé
Profiling microRNA (miRNA) expression is of widespread interest given the critical role of miRNAs in many cellular functions. Profiling can be achieved via hybridization-based (microarrays), sequencing-based, or amplification-based (quantitative reverse transcription-PCR, qPCR) technologies. Among these, microarrays face the significant challenge of accurately distinguishing between mature and immature miRNA forms, and different vendors have developed different methods to meet this challenge. Here we measure differential miRNA expression using the Affymetrix, Agilent, and Illumina microarray platforms, as well as qPCR (Applied Biosystems) and ultra high-throughput sequencing (Illumina). We show that the differential expression measurements are more divergent when the three types of microarrays are compared than when the Agilent microarray, qPCR, and sequencing technology measurements are compared, which exhibit a good overall concordance.
Mots-clé
Brain/metabolism, Gene Expression Profiling/methods, Humans, MicroRNAs/biosynthesis, MicroRNAs/genetics, Myocardium/metabolism, Oligonucleotide Array Sequence Analysis/methods, Polymerase Chain Reaction/methods, Regression Analysis, Sequence Analysis, RNA/methods
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/03/2011 11:34
Dernière modification de la notice
20/08/2019 15:30
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