Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles.

Détails

Ressource 1Télécharger: BIB_BC7848C64B2B.P001.pdf (9428.12 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_BC7848C64B2B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles.
Périodique
Nucleic Acids Research
Auteur(s)
Demurtas D., Amzallag A., Rawdon E.J., Maddocks J.H., Dubochet J., Stasiak A.
ISSN
1362-4962[electronic], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
37
Numéro
9
Pages
2882-2893
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
We use cryo-electron microscopy (cryo-EM) to study the 3D shapes of 94-bp-long DNA minicircles and address the question of whether cyclization of such short DNA molecules necessitates the formation of sharp, localized kinks in DNA or whether the necessary bending can be redistributed and accomplished within the limits of the elastic, standard model of DNA flexibility. By comparing the shapes of covalently closed, nicked and gapped DNA minicircles, we conclude that 94-bp-long covalently closed and nicked DNA minicircles do not show sharp kinks while gapped DNA molecules, containing very flexible single-stranded regions, do show sharp kinks. We corroborate the results of cryo-EM studies by using Bal31 nuclease to probe for the existence of kinks in 94-bp-long minicircles.
Mots-clé
Algorithms, Cryoelectron Microscopy, Cyclization, DNA, Circular/chemistry, DNA, Circular/metabolism, Endodeoxyribonucleases/metabolism, Nucleic Acid Conformation
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/06/2009 15:34
Dernière modification de la notice
20/08/2019 16:30
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