The yeast acid phosphatase can enter the secretory pathway without its N-terminal signal sequence

Détails

ID Serval
serval:BIB_BC74F20BAF04
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The yeast acid phosphatase can enter the secretory pathway without its N-terminal signal sequence
Périodique
Molecular and Cellular Biology
Auteur(s)
Silve  S., Monod  M., Hinnen  A., Haguenauer-Tsapis  R.
ISSN
0270-7306 (Print)
Statut éditorial
Publié
Date de publication
09/1987
Volume
7
Numéro
9
Pages
3306-14
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall glycoprotein that follows the yeast secretory pathway. We used in vitro mutagenesis to construct a deletion (delta SP) including the entire signal sequence and four amino acids of the mature sequence of APase. An APase-deficient yeast strain was transformed with a high-copy-number plasmid carrying the PHO5/delta SP gene. When expressed in vivo, the PHO5/delta SP gene product accumulated predominantly as an inactive, unglycosylated form located inside the cell. A large part of this unglycosylated precursor underwent proteolytic degradation, but up to 30% of it was translocated, core glycosylated, and matured by the addition of mannose residues, before reaching the cell wall. It appears, therefore, that the signal sequence is important for efficient translocation and core glycosylation of yeast APase but that it is not absolutely necessary for entry of the protein into the yeast secretory pathway. mRNA obtained by in vitro transcription of PHO5 and PHO5/delta SP genes were translated in vitro in the presence of either reticulocyte lysate and dog pancreatic microsomes or yeast lysate and yeast microsomes. The PHO5 gene product was translocated and core glycosylated in the heterologous system and less efficiently in the homologous system. We were not able to detect any translocation or glycosylation of PHO5/delta SP gene product in the heterologous system, but a very small amount of core suppression of glycosylated material could be evidenced in the homologous system.
Mots-clé
Acid Phosphatase/genetics/*secretion Amino Acid Sequence Animals Biological Transport Chromosome Deletion Dogs Glycoproteins/*secretion Immunologic Techniques Kinetics Microsomes/metabolism Molecular Weight Protein Processing, Post-Translational Protein Sorting Signals/*physiology Saccharomyces cerevisiae/*enzymology Structure-Activity Relationship
Pubmed
Web of science
Création de la notice
25/01/2008 17:47
Dernière modification de la notice
20/08/2019 16:30
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