Increased hepatic fatty acid polyunsaturation precedes ectopic lipid deposition in the liver in adaptation to high-fat diets in mice.

Détails

ID Serval
serval:BIB_BC5FBDB38ABA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Increased hepatic fatty acid polyunsaturation precedes ectopic lipid deposition in the liver in adaptation to high-fat diets in mice.
Périodique
Magma
Auteur(s)
Soares A.F., Duarte JMN, Gruetter R.
ISSN
1352-8661 (Electronic)
ISSN-L
0968-5243
Statut éditorial
Publié
Date de publication
04/2018
Peer-reviewed
Oui
Volume
31
Numéro
2
Pages
341-354
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
We monitored hepatic lipid content (HLC) and fatty acid (FA) composition in the context of enhanced lipid handling induced by a metabolic high-fat diet (HFD) challenge and fasting.
Mice received a control diet (10% of kilocalories from fat, N = 14) or an HFD (45% or 60% of kilocalories from fat, N = 10 and N = 16, respectively) for 26 weeks. A subset of five mice receiving an HFD (60% of kilocalories from fat) were switched to the control diet for the final 7 weeks. At nine time points, magnetic resonance spectroscopy was performed in vivo at 14.1 T, interleaved with glucose tolerance tests.
Glucose intolerance promptly developed with the HFD, followed by a progressive increase of fasting insulin level, simultaneously with that of HLC. These metabolic defects were normalized by dietary reversal. HFD feeding immediately increased polyunsaturation of hepatic FA, before lipid accumulation. Fasting-induced changes in hepatic lipids (increased HLC and FA polyunsaturation, decreased FA monounsaturation) in control-diet-fed mice were not completely reproduced in HFD-fed mice, not even after dietary reversal.
A similar adaptation of hepatic lipids to both fasting and an HFD suggests common mechanisms of lipid trafficking from adipose tissue to the liver. Altered hepatic lipid handling with fasting indicates imperfect metabolic recovery from HFD exposure.
Mots-clé
Adipose Tissue/metabolism, Animals, Diet, High-Fat, Fatty Acids/chemistry, Fatty Acids, Unsaturated/chemistry, Glucose Tolerance Test, Insulin/blood, Lipids/chemistry, Liver/metabolism, Magnetic Resonance Spectroscopy, Male, Mice, Mice, Inbred C57BL, Obesity/metabolism, Phenotype, Insulin resistance, Liver, Nuclear Magnetic Resonance, Nutrition, Obesity, Polyunsaturated fat
Pubmed
Web of science
Création de la notice
26/10/2017 16:25
Dernière modification de la notice
20/08/2019 16:30
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