Solute carrier family 12 member 2 as a proteomic and histological biomarker of dysplasia and neoplasia in ulcerative colitis.

Détails

ID Serval
serval:BIB_BC5DB4C1FDFB
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Lettre (letter): communication adressée à l'éditeur.
Collection
Publications
Institution
Titre
Solute carrier family 12 member 2 as a proteomic and histological biomarker of dysplasia and neoplasia in ulcerative colitis.
Périodique
Journal of Crohn's & colitis
Auteur⸱e⸱s
Merli A.M., Vieujean S., Massot C., Blétard N., Quesada Calvo F., Baiwir D., Mazzucchelli G., Servais L., Wéra O., Oury C., de Leval L., Sempoux C., Manzini R., Bluemel S., Scharl M., Rogler G., De Pauw E., Coimbra Marques C., Colard A., Vijverman A., Delvenne P., Louis E., Meuwis M.A.
ISSN
1876-4479 (Electronic)
ISSN-L
1873-9946
Statut éditorial
Publié
Date de publication
28/02/2021
Peer-reviewed
Oui
Volume
15
Numéro
2
Pages
287–298
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Ulcerative colitis (UC) patients have a greater risk of developing colorectal cancer through inflammation-dysplasia-carcinoma sequence of transformation. The histopathological diagnosis of dysplasia is therefore of critical clinical relevance, but dysplasia may be difficult to distinguish from inflammatory changes.
A proteomic pilot study on 5 UC colorectal dysplastic patients highlighted proteins differentially distributed between paired dysplastic, inflammatory and normal tissues. The best candidate marker was selected and immunohistochemistry confirmation was performed on AOM/DSS mouse model lesions, 37 UC dysplasia, 14 UC cancers, 23 longstanding UC, 35 sporadic conventional adenomas, 57 sporadic serrated lesions and 82 sporadic colorectal cancers.
Differential proteomics found 11 proteins significantly more abundant in dysplasia compared to inflammation, including Solute carrier family 12 member 2 (SLC12A2) which was confidently identified with 8 specific peptides and was below the limit of quantitation in both inflammatory and normal colon. SLC12A2 immunohistochemical analysis confirmed the discrimination of preneoplastic and neoplastic lesions from inflammatory lesions in mice, UC and in sporadic contexts. A specific SLC12A2 staining pattern termed "loss of gradient" reached 89% sensitivity, 95% specificity and 92% accuracy for UC-dysplasia diagnosis together with an inter-observer agreement of 95.24% (multirater κfree of 0.90; IC95%: 0.78 - 1.00). Such discrimination could not be obtained by Ki67 staining. This specific pattern was also associated with sporadic colorectal adenomas and cancers.
We found a specific SLC12A2 immunohistochemical staining pattern in precancerous and cancerous colonic UC-lesions which could be helpful for diagnosing dysplasia and cancer in UC and non-UC patients.
Mots-clé
SLC12A2, colorectal neoplasia, dysplasia associated to inflammation
Pubmed
Web of science
Création de la notice
15/09/2020 14:40
Dernière modification de la notice
27/05/2023 6:50
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