Glycans on secretory component participate in innate protection against mucosal pathogens.

Détails

ID Serval
serval:BIB_BBBC9A029B59
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Glycans on secretory component participate in innate protection against mucosal pathogens.
Périodique
Journal of Biological Chemistry
Auteur⸱e⸱s
Perrier C., Sprenger N., Corthésy B.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
05/2006
Peer-reviewed
Oui
Volume
281
Numéro
20
Pages
14280-14287
Langue
anglais
Notes
Publication types: Journal Article
Résumé
In mucosal secretions, secretory component (SC) is found either free or bound to polymeric IgA within the secretory IgA complex. SC displays numerous and various glycans, which are potential ligands for bacterial compounds. We first established that human SC (hSC) purified from colostrum (hSCcol) or produced in Chinese hamster ovary cells (hSCrec) exhibits the same lectin reactivity. Both forms bind to Clostridium difficile toxin A and functionally protect polarized Caco-2 cell monolayers from the cytopathic effect of the toxin. The interaction is mediated by glycans present on hSC and involves galactose and sialic acid residues. hSCcol and hSCrec were also shown to bind enteropathogenic Escherichia coli adhesin intimin and to inhibit its infectivity on HEp-2 cells in a glycan-dependent manner as well. SC remained operative in the context of the whole secretory IgA molecule and can therefore enhance its Fab-mediated neutralizing properties. On the contrary, hSC did not interact with three different strains of rotavirus (RF, RRV, and SA11). Accordingly, infection of target MA104 cells with these rotavirus strains was not reduced in the presence of either form of hSC tested. Although not a universal mechanism, these findings identify hSC as a microbial scavenger contributing to the antipathogenic arsenal that protects the body epithelial surfaces.
Mots-clé
Animals, Bacterial Adhesion, Bacterial Toxins/chemistry, CHO Cells, Caco-2 Cells, Cell Line, Tumor, Cricetinae, Enterotoxins/chemistry, Escherichia coli/metabolism, Humans, Mucous Membrane/metabolism, Polysaccharides/chemistry, Protein Structure, Tertiary, Recombinant Fusion Proteins/chemistry, Rotavirus/genetics, Rotavirus/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:53
Dernière modification de la notice
20/08/2019 15:29
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