CD3 delta establishes a functional link between the T cell receptor and CD8.

Détails

ID Serval
serval:BIB_BB6E67E4C733
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CD3 delta establishes a functional link between the T cell receptor and CD8.
Périodique
Journal of Biological Chemistry
Auteur(s)
Doucey M.A., Goffin L., Naeher D., Michielin O., Baumgärtner P., Guillaume P., Palmer E., Luescher I.F.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
278
Numéro
5
Pages
3257-3264
Langue
anglais
Résumé
T cells expressing T cell receptor (TCR) complexes that lack CD3 delta, either due to deletion of the CD3 delta gene, or by replacement of the connecting peptide of the TCR alpha chain, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. Because the same defects have been observed in mice expressing no CD8 beta or tailless CD8 beta, we examined whether CD3 delta serves to couple TCR.CD3 with CD8. To this end we used T cell hybridomas and transgenic mice expressing the T1 TCR, which recognizes a photoreactive derivative of the PbCS 252-260 peptide in the context of H-2K(d). We report that, in thymocytes and hybridomas expressing the T1 TCR.CD3 complex, CD8 alpha beta associates with the TCR. This association was not observed on T1 hybridomas expressing only CD8 alpha alpha or a CD3 delta(-) variant of the T1 TCR. CD3 delta was selectively co-immunoprecipitated with anti-CD8 antibodies, indicating an avid association of CD8 with CD3 delta. Because CD8 alpha beta is a raft constituent, due to this association a fraction of TCR.CD3 is raft-associated. Cross-linking of these TCR-CD8 adducts results in extensive TCR aggregate formation and intracellular calcium mobilization. Thus, CD3 delta couples TCR.CD3 with raft-associated CD8, which is required for effective activation and positive selection of CD8(+) T cells.
Mots-clé
Affinity Labels, Amino Acid Sequence, Animals, Antigens, CD3/chemistry, Antigens, CD3/genetics, Antigens, CD8/immunology, Calcium Signaling/physiology, Genetic Variation, Hybridomas/microbiology, Lymphocyte Activation, Mast-Cell Sarcoma, Membrane Microdomains/immunology, Mice, Mice, Transgenic, Models, Molecular, Molecular Sequence Data, Peptide Fragments/immunology, Protein Conformation, Receptor-CD3 Complex, Antigen, T-Cell/immunology, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, Sequence Alignment, T-Lymphocytes/immunology, Transfection, Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:20
Dernière modification de la notice
20/08/2019 15:29
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