Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP
Détails
ID Serval
serval:BIB_BB24B8484DC5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP
Périodique
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
06/1989
Volume
86
Numéro
11
Pages
4117-21
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Résumé
The mRNA encoding the mouse homolog of C/EBP, a rat DNA-binding protein that participates in activating a number of genes in hepatocytes, is present in liver cells at a far higher concentration than in most other cells, including spleen, kidney, muscle, and the majority of the brain. However, fat cells and intestinal cells contain 25-50% as much mRNA as liver cells. "Run-on" experiments show that the basis for the restricted cellular distribution of the mouse C/EBP mRNA is transcriptional regulation of the gene. We also show that disruption of cell-cell contacts incident to liver cell dispersion results in a prompt and extensive reduction in mouse C/EBP transcription as we had earlier shown to be the case for a group of 10 genes transcribed in a hepatocyte-specific fashion. In contrast, breaking cell contacts and plating the hepatocytes in culture leads to a prolonged increase in transcription of the Jun-B gene that encodes a widely distributed transcription factor. These results illustrate that the regulation of expression of a mammalian regulatory protein with limited tissue distribution is controlled at the level of transcription and depends on cell contacts.
Mots-clé
Animals
CCAAT-Enhancer-Binding Proteins
Cell Nucleus/metabolism
Cells, Cultured
Cloning, Molecular
DNA/genetics
DNA-Binding Proteins/*genetics
*Gene Expression Regulation
*Genes
Liver/metabolism
Mice
Nuclear Proteins/*genetics
Organ Specificity
RNA, Messenger/analysis/genetics
Rats
*Transcription, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 9:41
Dernière modification de la notice
20/08/2019 16:29