Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP

Détails

ID Serval
serval:BIB_BB24B8484DC5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Xanthopoulos  K. G., Mirkovitch  J., Decker  T., Kuo  C. F., Darnell, J. E., Jr. 
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
06/1989
Volume
86
Numéro
11
Pages
4117-21
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Résumé
The mRNA encoding the mouse homolog of C/EBP, a rat DNA-binding protein that participates in activating a number of genes in hepatocytes, is present in liver cells at a far higher concentration than in most other cells, including spleen, kidney, muscle, and the majority of the brain. However, fat cells and intestinal cells contain 25-50% as much mRNA as liver cells. "Run-on" experiments show that the basis for the restricted cellular distribution of the mouse C/EBP mRNA is transcriptional regulation of the gene. We also show that disruption of cell-cell contacts incident to liver cell dispersion results in a prompt and extensive reduction in mouse C/EBP transcription as we had earlier shown to be the case for a group of 10 genes transcribed in a hepatocyte-specific fashion. In contrast, breaking cell contacts and plating the hepatocytes in culture leads to a prolonged increase in transcription of the Jun-B gene that encodes a widely distributed transcription factor. These results illustrate that the regulation of expression of a mammalian regulatory protein with limited tissue distribution is controlled at the level of transcription and depends on cell contacts.
Mots-clé
Animals CCAAT-Enhancer-Binding Proteins Cell Nucleus/metabolism Cells, Cultured Cloning, Molecular DNA/genetics DNA-Binding Proteins/*genetics *Gene Expression Regulation *Genes Liver/metabolism Mice Nuclear Proteins/*genetics Organ Specificity RNA, Messenger/analysis/genetics Rats *Transcription, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 8:41
Dernière modification de la notice
20/08/2019 15:29
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