Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron

Détails

ID Serval
serval:BIB_BACE2BD10E40
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron
Périodique
American Journal of Physiology. Renal Physiology
Auteur(s)
Loffing  J., Loffing-Cueni  D., Valderrabano  V., Klausli  L., Hebert  S. C., Rossier  B. C., Hoenderop  J. G., Bindels  R. J., Kaissling  B.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
12/2001
Peer-reviewed
Oui
Volume
281
Numéro
6
Pages
F1021-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
The organization of Na(+) and Ca(2+) transport pathways along the mouse distal nephron is incompletely known. We revealed by immunohistochemistry a set of Ca(2+) and Na(+) transport proteins along the mouse distal convolution. The thiazide-sensitive Na(+)-Cl(-) cotransporter (NCC) characterized the distal convoluted tubule (DCT). The amiloride-sensitive epithelial Na(+) channel (ENaC) colocalized with NCC in late DCT (DCT2) and extended to the downstream connecting tubule (CNT) and collecting duct (CD). In early DCT (DCT1), the basolateral Ca(2+)-extruding proteins [Na(+)/Ca(2+) exchanger (NCX), plasma membrane Ca(2+)-ATPase (PCMA)] and the cytoplasmic Ca(2+)-binding protein calbindin D(28K) (CB) were found at very low levels, whereas the cytoplasmic Ca(2+)/Mg(2+)-binding protein parvalbumin was highly abundant. NCX, PMCA, and CB prevailed in DCT2 and CNT, where we located the apical epithelial Ca(2+) channel (ECaC1). Its subcellular localization changed from apical in DCT2 to exclusively cytoplasmic at the end of CNT. NCX and PMCA decreased in parallel with the fading of ECaC1 in the apical membrane. All three of them were undetectable in CD. These findings disclose DCT2 and CNT as major sites for transcellular Ca(2+) transport in the mouse distal nephron. Cellular colocalization of Ca(2+) and Na(+) transport pathways suggests their mutual interactions in transport regulation.
Mots-clé
Animals Calcium/*metabolism Calcium Channels/analysis/immunology Calcium-Binding Protein, Vitamin D-Dependent/analysis/immunology Calcium-Transporting ATPases/analysis/immunology Carrier Proteins/*analysis/immunology Cation Transport Proteins Epithelial Sodium Channel Female Immunohistochemistry Ion Transport Kidney Tubules, Distal/chemistry/*metabolism Mice Models, Biological Parvalbumins/analysis/immunology Plasma Membrane Calcium-Transporting ATPases *Receptors, Drug Sodium/*metabolism Sodium Channels/analysis/immunology Sodium Chloride Symporters Sodium-Calcium Exchanger/analysis/immunology *Symporters TRPV Cation Channels
Pubmed
Web of science
Création de la notice
24/01/2008 13:01
Dernière modification de la notice
20/08/2019 15:28
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