In vitro Cortical Network Firing is Homeostatically Regulated: A Model for Sleep Regulation.

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_BACB5342B86D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro Cortical Network Firing is Homeostatically Regulated: A Model for Sleep Regulation.
Périodique
Scientific Reports
Auteur⸱e⸱s
Saberi-Moghadam S., Simi A., Setareh H., Mikhail C., Tafti M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
8
Numéro
1
Pages
6297
Langue
anglais
Résumé
Prolonged wakefulness leads to a homeostatic response manifested in increased amplitude and number of electroencephalogram (EEG) slow waves during recovery sleep. Cortical networks show a slow oscillation when the excitatory inputs are reduced (during slow wave sleep, anesthesia), or absent (in vitro preparations). It was recently shown that a homeostatic response to electrical stimulation can be induced in cortical cultures. Here we used cortical cultures grown on microelectrode arrays and stimulated them with a cocktail of waking neuromodulators. We found that recovery from stimulation resulted in a dose-dependent homeostatic response. Specifically, the inter-burst intervals decreased, the burst duration increased, the network showed higher cross-correlation and strong phasic synchronized burst activity. Spectral power below <1.75 Hz significantly increased and the increase was related to steeper slopes of bursts. Computer simulation suggested that a small number of clustered neurons could potently drive the behavior of the network both at baseline and during recovery. Thus, this in vitro model appears valuable for dissecting network mechanisms of sleep homeostasis.

Pubmed
Web of science
Open Access
Oui
Création de la notice
26/04/2018 17:59
Dernière modification de la notice
20/08/2019 15:28
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