K-ras mutations and p53 alterations in neoplastic and nonneoplastic lesions associated with longstanding ulcerative colitis

Détails

ID Serval
serval:BIB_BAA528E1E126
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
K-ras mutations and p53 alterations in neoplastic and nonneoplastic lesions associated with longstanding ulcerative colitis
Périodique
American Journal of Pathology
Auteur(s)
Chaubert  P., Benhattar  J., Saraga  E., Costa  J.
ISSN
0002-9440 (Print)
Statut éditorial
Publié
Date de publication
1994
Volume
144
Numéro
4
Pages
767-775
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
We have analyzed K-ras mutations and p53 alterations in 39 tumor and nontumor samples taken from nine patients with longstanding ulcerative colitis and colorectal carcinoma. Two of nine invasive carcinomas contained a K-ras mutation. By a combination of immunohistochemistry and single-strand conformation polymorphism analysis, p53 alterations were found in three of nine carcinomas. Five of 13 dysplastic lesions harbored a mutated K-ras gene, even in the absence of detectable changes in associated invasive tumors. One single focus of dysplastic mucosa harbored concomitant K-ras and p53 gene alterations. In two patients, a K-ras mutation was detected in epithelial lesions considered to be devoid of malignant potential (villous regeneration, active colitis). Our results indicate that: 1) the prevalence of K-ras and p53 genetic alterations found in ulcerative colitis-associated colonic carcinomas appears to be lower than in sporadic carcinomas; 2) K-ras mutations can be detected in dysplasia, villous regeneration, and active colitis and affect a subpopulation of the cells composing the lesions; 3) diverse genetic alterations can be detected in the same patient and the dysplastic lesions can exhibit a different genotype than the carcinomas; and 4) at least part of active colitis and villous regeneration lesions should be considered as preneoplastic in ulcerative colitis
Mots-clé
Adult/Aged/Base Sequence/Carcinoma/etiology/metabolism/Pathology/Colitis,Ulcerative/complications/Colorectal Neoplasms/Female/Humans/Immunoenzyme Techniques/Male/Middle Aged/Molecular Sequence Data/Mutation/Oncogene Protein p21(ras)/genetics/Polymerase Chain Reaction/Tumor Suppressor Protein p53/Research
Pubmed
Web of science
Création de la notice
29/01/2008 19:33
Dernière modification de la notice
20/08/2019 16:28
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