Dopamine receptors on dispersed bovine anterior pituitary cells

Détails

ID Serval
serval:BIB_B9D69146B2DD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dopamine receptors on dispersed bovine anterior pituitary cells
Périodique
Neuroendocrinology
Auteur(s)
Ramsdell  J. S., Monnet F., Weiner  R. I.
ISSN
0028-3835
Statut éditorial
Publié
Date de publication
1985
Peer-reviewed
Oui
Volume
41
Numéro
2
Pages
169-176
Langue
anglais
Résumé
The dopamine antagonist [3H]-domperidone-[3H]-DOM-bound to a single class of high-affinity (Kd = 1.24 +/- 0.14 nM) and saturable receptors on dispersed bovine anterior pituitary (AP) cells. The binding of [3H]-DOM was stereoselective and reversible with agonists and antagonists. Dopamine competitions for [3H]-DOM binding modeled best for a single site consistent with an interaction with a homogeneous population of receptors. The mean number of specific binding sites labeled by [3H]-DOM was 53,000 per cell in dispersed AP cells consisting of 42% lactotrophs. Dispersed bovine AP cells attached to extracellular matrix within 3 h, and prolactin secretion from these cells was effectively inhibited by dopamine. Several observations suggested that [3H]-DOM-labeled receptors on dispersed bovine AP cells were restricted to the outer plasma membrane and not internalized. These included (1) the rapid and complete dissociation of specific [3H]-DOM binding; (2) the ability of treatment with acid or proteolytic enzymes to entirely remove specifically bound [3H]-DOM, and (3) the lack of effect of metabolic inhibitors on specific [3H]-DOM binding.
Mots-clé
Animals Binding, Competitive Cattle Cell Membrane/metabolism Domperidone/metabolism Dopamine/metabolism Immunoenzyme Techniques Pituitary Gland, Anterior/*analysis Radioligand Assay Receptors, Dopamine/*analysis/metabolism
Pubmed
Web of science
Création de la notice
10/02/2009 13:05
Dernière modification de la notice
20/08/2019 15:27
Données d'usage