OCA-B is a functional analog of VP16 but targets a separate surface of the Oct-1 POU domain.

Détails

ID Serval
serval:BIB_B9BB957CFD07
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
OCA-B is a functional analog of VP16 but targets a separate surface of the Oct-1 POU domain.
Périodique
Molecular and Cellular Biology
Auteur(s)
Babb R., Cleary M.A., Herr W.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
12/1997
Volume
17
Numéro
12
Pages
7295-7305
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
OCA-B is a B-cell-specific coregulator of the broadly expressed POU domain transcription factor Oct-1. OCA-B associates with the Oct-1 POU domain, a bipartite DNA-binding structure containing a POU-specific (POU[S]) domain joined by a flexible linker to a POU homeodomain (POU[H]). Here, we show that OCA-B alters the activity of Oct-1 in two ways. It provides a transcriptional activation domain which, unlike Oct-1, activates an mRNA-type promoter effectively, and it stabilizes Oct-1 on the Oct-1-responsive octamer sequence ATGCAAAT. These properties of OCA-B parallel those displayed by the herpes simplex virus Oct-1 coregulator VP16. OCA-B, however, interacts with a different surface of the DNA-bound Oct-1 POU domain, interacting with both the POU(S) and POU(H) domains and the center of the ATGCAAAT octamer sequence. The OCA-B and VP16 interactions with the Oct-1 POU domain are sufficiently different to permit OCA-B and VP16 to bind the Oct-1 POU domain simultaneously. These results emphasize the structural versatility of the Oct-1 POU domain in its interaction with coregulators.
Mots-clé
Amino Acid Sequence, B-Lymphocytes/metabolism, Base Sequence, Binding Sites/genetics, DNA/genetics, DNA/metabolism, DNA Primers/genetics, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Hela Cells, Herpes Simplex Virus Protein Vmw65/genetics, Herpes Simplex Virus Protein Vmw65/metabolism, Host Cell Factor C1, Humans, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Octamer Transcription Factor-1, Polymerase Chain Reaction, Promoter Regions, Genetic, Protein Conformation, RNA, Messenger/genetics, Trans-Activators/chemistry, Trans-Activators/genetics, Transcription Factors/chemistry, Transcription Factors/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 15:27
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