International collaborative proficiency study of Human Papillomavirus type 16 serology.

Détails

ID Serval
serval:BIB_B9B066FBBAD8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
International collaborative proficiency study of Human Papillomavirus type 16 serology.
Périodique
Vaccine
Auteur(s)
Eklund C., Unger E.R., Nardelli-Haefliger D., Zhou T., Dillner J.
ISSN
1873-2518 (Electronic)
ISSN-L
0264-410X
Statut éditorial
Publié
Date de publication
2012
Volume
30
Numéro
2
Pages
294-299
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
We performed an international proficiency study of Human Papillomavirus (HPV) type 16 serology. A common methodology for serology based on virus-like particle (VLP) ELISA was used by 10 laboratories in 6 continents. The laboratories used the same VLP reference reagent, which was selected as the most stable, sensitive and specific VLP preparation out of VLPs donated from 5 different sources. A blinded proficiency panel consisting of 52 serum samples from women with PCR-verified HPV 16-infection, 11 control serum samples from virginal women and the WHO HPV 16 International Standard (IS) serum were distributed. The mean plus 3 standard deviations of the negative control serum samples was the most generally useful "cut-off" criterion for distinguishing positive and negative samples. Using sensitivity of at least 50% and a specificity of 100% as proficiency criteria, 6/10 laboratories were proficient. In conclusion, an international Standard Operating Procedure for HPV serology, an international reporting system in International Units (IU) and a common "cut-off" criterion have been evaluated in an international HPV serology proficiency study.
Pubmed
Web of science
Création de la notice
06/03/2012 19:29
Dernière modification de la notice
20/08/2019 15:27
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