Isolation and sequence of rat peptide YY and neuropeptide Y

Détails

ID Serval
serval:BIB_B9ACE1C70FBF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Isolation and sequence of rat peptide YY and neuropeptide Y
Périodique
Regulatory Peptides
Auteur(s)
Corder  R., Gaillard  R. C., Bohlen  P.
ISSN
0167-0115 (Print)
Statut éditorial
Publié
Date de publication
06/1988
Volume
21
Numéro
3-4
Pages
253-61
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Rat peptide YY and rat neuropeptide Y have been isolated in parallel from colon and brain extracts respectively, using salt fractionation, gel filtration chromatography, cation-exchange HPLC, and reverse phase phenyl-silica HPLC. Immunoreactivity was identified using a combination of 3 NPY immunoassays which exhibit differing cross-reactivities for PYY (90%, less than 0.01% and 30% respectively). The yield at the final purification step was 1.2 nmol rPYY and 0.5 nmol rNPY. Half of each purified peptide was subjected to complete microsequence analysis. This showed that while rat NPY was structurally identical to human NPY, the sequence of PYY from rat colon was the same as porcine PYY isolated from extracts of duodenum.
Mots-clé
Amino Acid Sequence Animals Chromatography, Gel Chromatography, High Pressure Liquid Gastrointestinal Hormones/*isolation & purification Immunoassay/methods Molecular Sequence Data Neuropeptide Y/*isolation & purification Peptide YY Peptides/*isolation & purification Radiometry Rats
Pubmed
Web of science
Création de la notice
15/02/2008 16:57
Dernière modification de la notice
20/08/2019 15:27
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