Assessment of the Accuprobe Listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths

Détails

ID Serval
serval:BIB_B9A73FD04E53
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Assessment of the Accuprobe Listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths
Périodique
Applied and Environmental Microbiology
Auteur(s)
Ninet  B., Bannerman  E., Bille  J.
ISSN
0099-2240
Statut éditorial
Publié
Date de publication
12/1992
Peer-reviewed
Oui
Volume
58
Numéro
12
Pages
4055-9
Notes
Comparative Study
Journal Article --- Old month value: Dec
Résumé
The Accuprobe Listeria monocytogenes Culture Identification Reagent Kit, a nonradioactive probe, was evaluated as a colony confirmation test and in different selective or nonselective enrichment broths. The probe was 100% sensitive and 100% specific when applied to isolated colonies. The minimal detection limit in physiological saline was established to be about 10(5) CFU of L. monocytogenes. Hybridization done directly in broths seeded with L. monocytogenes showed variable results. Three nonselective broths (Todd-Hewitt broth, brain heart infusion broth, and tryptic soy broth) and one selective broth (FDA) gave positive reactions at an inoculum of 5 x 10(6) CFU, whereas two other selective broths (UVM, and PALCAMY) gave negative reactions with up to 10(8) and 10(9) CFU. In FDA broth, the level of detection of L. monocytogenes was not modified by the presence of other organisms in mixed cultures.
Mots-clé
*Bacteriological Techniques Culture Media DNA Probes Evaluation Studies as Topic Food Microbiology Food Poisoning/diagnosis Humans Listeria Infections/diagnosis Listeria monocytogenes/genetics/growth & development/*isolation & purification *Molecular Probe Techniques
Pubmed
Web of science
Création de la notice
11/02/2008 12:40
Dernière modification de la notice
20/08/2019 15:27
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