Transcription factor activity rhythms and tissue-specific chromatin interactions explain circadian gene expression across organs.

Détails

ID Serval
serval:BIB_B99C34A1E160
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcription factor activity rhythms and tissue-specific chromatin interactions explain circadian gene expression across organs.
Périodique
Genome research
Auteur(s)
Yeung Jake, Mermet Jerome, Jouffe Celine, Marquis Julien, Charpagne Aline, Gachon Frederic, Naef Felix
Statut éditorial
Publié
Date de publication
02/2018
Volume
28
Numéro
2
Pages
182-191
Langue
anglais
Résumé
Temporal control of physiology requires the interplay between gene networks involved in daily timekeeping and tissue function across different organs. How the circadian clock interweaves with tissue-specific transcriptional programs is poorly understood. Here, we dissected temporal and tissue-specific regulation at multiple gene regulatory layers by examining mouse tissues with an intact or disrupted clock over time. Integrated analysis uncovered two distinct regulatory modes underlying tissue-specific rhythms: tissue-specific oscillations in transcription factor (TF) activity, which were linked to feeding-fasting cycles in liver and sodium homeostasis in kidney; and colocalized binding of clock and tissue-specific transcription factors at distal enhancers. Chromosome conformation capture (4C-seq) in liver and kidney identified liver-specific chromatin loops that recruited clock-bound enhancers to promoters to regulate liver-specific transcriptional rhythms. Furthermore, this looping was remarkably promoter-specific on the scale of less than 10 kilobases (kb). Enhancers can contact a rhythmic promoter while looping out nearby nonrhythmic alternative promoters, confining rhythmic enhancer activity to specific promoters. These findings suggest that chromatin folding enables the clock to regulate rhythmic transcription of specific promoters to output temporal transcriptional programs tailored to different tissues.
Mots-clé
Animals, Mice, Promoter Regions, Genetic, Chromatin/genetics, Circadian Clocks/*genetics, Circadian Rhythm/*genetics, Enhancer Elements, Genetic/*genetics, Gene Expression Regulation/genetics, Kidney/metabolism, Liver/metabolism, Organ Specificity/genetics, Transcription Factors/*genetics
Pubmed
Création de la notice
19/02/2020 12:23
Dernière modification de la notice
20/02/2020 6:26
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