Predominant basolateral proteolytic processing of prosomatostatin into somatostatin-28 in polarized LLC-PK1 cells

Détails

ID Serval
serval:BIB_B99273FB983B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Predominant basolateral proteolytic processing of prosomatostatin into somatostatin-28 in polarized LLC-PK1 cells
Périodique
Neuropeptides
Auteur⸱e⸱s
Brakch  N., Yang  X. F., Crine  P., Cohen  P., Boileau  G.
ISSN
0143-4179 (Print)
Statut éditorial
Publié
Date de publication
10/1997
Volume
31
Numéro
5
Pages
393-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Polarized epithelial cells secrete specific proteins through their apical or basolateral membrane. In the present study, we have expressed the human prosomatostatin cDNA in the pig kidney epithelial cell line (LLC-PK1) and monitored the processing and release of the somatostatin-related peptides. Analysis by high-performance liquid chromatography and radioimmunoassay of the somatostatin-related peptides synthesized by the transfected cells showed that the LLC-PK1 cells released prosomatostatin and somatostatin-28 (S-28) in the culture medium. Furthermore, when the cells were polarized, we observed release of prosomatostatin from both membrane domains (apical and basolateral), while liberation of S-28 was mostly from the basolateral side. This observation suggests that, in these cells, the proprotein convertase(s) responsible for prosomatostatin processing is(are) associated with the basolateral secretory pathway.
Mots-clé
Animals Basement Membrane/metabolism Cell Polarity/*physiology Culture Media Epithelial Cells/metabolism/ultrastructure Hydrolysis LLC-PK1 Cells Protein Precursors/*metabolism Somatostatin/*metabolism Somatostatin-28 Swine Transfection
Pubmed
Web of science
Création de la notice
28/01/2008 11:35
Dernière modification de la notice
20/08/2019 16:27
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