Arginine availability controls the N-methyl-D-aspartate-induced nitric oxide synthesis: involvement of a glial-neuronal arginine transfer.

Détails

ID Serval
serval:BIB_B93B56AF3368
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Arginine availability controls the N-methyl-D-aspartate-induced nitric oxide synthesis: involvement of a glial-neuronal arginine transfer.
Périodique
Journal of Neurochemistry
Auteur(s)
Grima G., Cuénod M., Pfeiffer S., Mayer B., Do K.Q.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
71
Numéro
5
Pages
2139-2144
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The neuronal nitric oxide (NO) synthase generates NO from arginine. NO mediates its physiological effects mainly by stimulating the synthesis of cyclic GMP. We have investigated the role of the arginine availability on the NMDA-induced cyclic GMP accumulation in immature rat brain slices. The effect of NMDA was blocked by the inhibitor of the NO synthase, N(G)-nitro-L-arginine, and by the antagonist of ionotropic non-NMDA receptors, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). This inhibition was not due to a direct interaction of CNQX with the NMDA receptor, and it was overcome by the presence of exogenously applied arginine. CNQX also blocked the NMDA-evoked release of [3H]arginine from cerebellar slices. Moreover, the arginine uptake inhibitor L-lysine reduced the cyclic GMP response to NMDA significantly. Therefore, the extracellular arginine availability, which is dependent on the activation of ionotropic non-NMDA receptors, determines the rate of the NO biosynthesis by the neuronal NO synthase. Together with the reported release of arginine from glial cells upon activation of glial ionotropic non-NMDA receptors and the predominant glial localization of arginine, these data provide the first evidence of an essential role of the arginine transfer from glial cells to neurons for the biosynthesis of NO.
Mots-clé
6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology, Animals, Arginine/physiology, Cerebellum/cytology, Cerebellum/metabolism, Cerebral Cortex/cytology, Cerebral Cortex/metabolism, Cyclic GMP/biosynthesis, Enzyme Inhibitors/pharmacology, Excitatory Amino Acid Antagonists/pharmacology, N-Methylaspartate/pharmacology, Neuroglia/metabolism, Neurons/metabolism, Nitric Oxide/biosynthesis, Nitroarginine/pharmacology, Rats, Rats, Inbred Strains
Pubmed
Web of science
Création de la notice
07/03/2014 10:38
Dernière modification de la notice
20/08/2019 16:27
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