Comparison of morphological and functional alterations of human saphenous veins after seven and fourteen days of ex vivo perfusion
Détails
ID Serval
serval:BIB_B8E8112F6233
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of morphological and functional alterations of human saphenous veins after seven and fourteen days of ex vivo perfusion
Périodique
European Surgical Research
ISSN
0014-312X (Print)
Statut éditorial
Publié
Date de publication
10/2004
Volume
36
Numéro
5
Pages
274-81
Notes
Comparative Study
In Vitro
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15359090 --- Old month value: Sep-Oct
In Vitro
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15359090 --- Old month value: Sep-Oct
Résumé
Intimal hyperplasia (IH) is a vessel wall remodeling process responsible of early failure after vascular surgery or endovascular interventions. An ex vivo perfusion was used to study human venous segments regarding functional, histomorphological, immunohistochemical and molecular alterations after 7 (group 1, n = 6) and 14 days (group 2, n = 6) of ex vivo perfusion. All vessel segments showed preserved smooth muscle function before and after perfusion. Histomorphometry revealed IH development which was more pronounced after 14 days rather than 7 days (p < 0.05). Expression of CD34, factor VIII, alpha-actin and MIB-1 was demonstrated in all segments from both groups indicating that muscular and endothelial integrity was preserved after ex vivo perfusion of up to 14 days. PAI-1 mRNA expression was significantly increased after perfusion (p < 0.05), suggesting that the endothelial fibrinolytic function may be modulated in this ex vivo perfusion model of human saphenous veins.
Mots-clé
Actins/metabolism
Antigens, CD34/metabolism
Blotting, Northern
Factor VIII/metabolism
Female
Humans
Hyperplasia
Immunohistochemistry
Ki-67 Antigen/metabolism
Male
Middle Aged
Nitroprusside/pharmacology
Norepinephrine/pharmacology
*Perfusion/adverse effects
Plasminogen Activator Inhibitor 1/metabolism
Saphenous Vein/drug effects/*pathology/*physiopathology
Time Factors
Tunica Intima/pathology
Vasoconstriction
Vasoconstrictor Agents/pharmacology
Vasodilation
Vasodilator Agents/pharmacology
Pubmed
Web of science
Création de la notice
29/01/2008 13:59
Dernière modification de la notice
20/08/2019 16:26