Single-cell damage elicits regional, nematode-restricting ethylene responses in roots.

Détails

ID Serval
serval:BIB_B8DC62E87575
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Single-cell damage elicits regional, nematode-restricting ethylene responses in roots.
Périodique
The EMBO journal
Auteur⸱e⸱s
Marhavý P., Kurenda A., Siddique S., Dénervaud Tendon V., Zhou F., Holbein J., Hasan M.S., Grundler F.M., Farmer E.E., Geldner N.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
15/05/2019
Peer-reviewed
Oui
Volume
38
Numéro
10
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Plants are exposed to cellular damage by mechanical stresses, herbivore feeding, or invading microbes. Primary wound responses are communicated to neighboring and distal tissues by mobile signals. In leaves, crushing of large cell populations activates a long-distance signal, causing jasmonate production in distal organs. This is mediated by a cation channel-mediated depolarization wave and is associated with cytosolic Ca <sup>2+</sup> transient currents. Here, we report that much more restricted, single-cell wounding in roots by laser ablation elicits non-systemic, regional surface potential changes, calcium waves, and reactive oxygen species (ROS) production. Surprisingly, laser ablation does not induce a robust jasmonate response, but regionally activates ethylene production and ethylene-response markers. This ethylene activation depends on calcium channel activities distinct from those in leaves, as well as a specific set of NADPH oxidases. Intriguingly, nematode attack elicits very similar responses, including membrane depolarization and regional upregulation of ethylene markers. Moreover, ethylene signaling antagonizes nematode feeding, delaying initial syncytial-phase establishment. Regional signals caused by single-cell wounding thus appear to constitute a relevant root immune response against small invaders.
Mots-clé
Animals, Arabidopsis/genetics, Arabidopsis/metabolism, Calcium Signaling/physiology, Cyclopentanes/metabolism, Ethylenes/biosynthesis, Ethylenes/metabolism, Host-Parasite Interactions/physiology, Nematoda/metabolism, Oxylipins/metabolism, Plant Roots/metabolism, Plants, Genetically Modified, Reactive Oxygen Species/metabolism, Single-Cell Analysis, Stress, Mechanical, Stress, Physiological/physiology, Time-Lapse Imaging, ethylene, nematodes, regional response, single‐cell laser ablation, surface depolarization
Pubmed
Web of science
Création de la notice
27/05/2019 16:35
Dernière modification de la notice
11/01/2020 6:16
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