Clusterin expression during fetal and postnatal CNS development in mouse

Détails

ID Serval
serval:BIB_B8994F508B11
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Clusterin expression during fetal and postnatal CNS development in mouse
Périodique
Neuroscience
Auteur(s)
Charnay Yves, Imhof Anouk, Vallet Philippe G., Hakkoum David, Lathuilière A., Poku N., Aronow Bruce, Kövari Enikö, Bouras Constantin, Giannakopoulos Panteleimon
ISSN
0306-4522
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
155
Numéro
3
Pages
714-724
Langue
anglais
Notes
SAPHIRID:70502
Résumé
Clusterin (or apolipoprotein J) is a widely distributed multifunctional glycoprotein involved in CNS plasticity and post-traumatic remodeling. Using biochemical and morphological approaches, we investigated the clusterin ontogeny in the CNS of wild-type (WT) mice and explored developmental consequences of clusterin gene knock-out in clusterin null (Clu-/-) mice. A punctiform expression of clusterin mRNA was detected through the hypothalamic region, neocortex and hippocampus at embryonic stages E14/E15. From embryonic stage E16 to the first week of the postnatal life, the vast majority of CNS neurons expressed low levels of clusterin mRNA. In contrast, a very strong hybridizing signal mainly localized in pontobulbar and spinal cord motor nuclei was observed from the end of the first postnatal week to adulthood. Astrocytes expressing clusterin mRNA were often detected through the hippocampus and neocortex in neonatal mice. Real-time polymerase chain amplification and clusterin-immunoreactivity dot-blot analyses indicated that clusterin levels paralleled mRNA expression. Comparative analyses between WT and Clu-/- mice during postnatal development showed no significant differences in brain weight, neuronal, synaptic and astrocyte markers as well myelin basic protein expression. However, quantitative estimation of large motor neuron populations in the facial nucleus revealed a significant deficit in motor cells (-16%) in Clu-/- compared with WT mice. Our data suggest that clusterin expression is already present in fetal life mainly in subcortical structures. Although the lack of this protein does not significantly alter basic aspects of the CNS development, it may have a negative impact on neuronal development in certain motor nuclei.
Pubmed
Web of science
Création de la notice
18/11/2008 11:22
Dernière modification de la notice
20/08/2019 15:26
Données d'usage