In vitro activity of CD4+ and CD8+ T lymphocytes from mice immunized with a synthetic malaria peptide

Détails

ID Serval
serval:BIB_B8670D15F269
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro activity of CD4+ and CD8+ T lymphocytes from mice immunized with a synthetic malaria peptide
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Renia  L., Marussig  M. S., Grillot  D., Pied  S., Corradin  G., Miltgen  F., Del Giudice  G., Mazier  D.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
09/1991
Volume
88
Numéro
18
Pages
7963-7
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 15
Résumé
In previous work, a T-helper epitope was mapped within the circumsporozoite protein of the murine malaria parasite Plasmodium yoelii. A 21-mer synthetic peptide corresponding to this epitope (amino acid positions 59-79; referred to as Py1) induced a specific T-cell proliferation in BALB/c and C57BL/6 mice and provided help for the production of antibodies to peptides from the repetitive region, (Gln-Gly-Pro-Gly-Ala-Pro)n, of the P. yoelii circumsporozoite protein when mice were immunized with the Py1 peptide conjugated to the repetitive peptide. Experiments were then designed to study the in vitro antiparasite efficacy of T cells elicited in vivo by peptide immunization. T-cell activity was evaluated on cultured hepatic stages of P. yoelii. Peptide immunizations led to the preferential activation of CD8+ T cells in BALB/c mice and of both CD4+ and CD8+ T cells in C57BL/6 mice. Parasite elimination was mediated directly by these cells and did not seem to be dependent on lymphokine secretion. These data suggest that peptide-primed CD4+ T cells as well as CD8+ T cells could be cytolytic for the hepatic phase of malaria parasites. The fact that the same peptide could activate different lymphocyte populations, depending on the strain of mouse, highlights the importance of a better understanding of the fine mechanisms behind the immune responses to synthetic peptides being tested for malaria vaccine development.
Mots-clé
Animals Antigens, CD8 Antigens, Differentiation, T-Lymphocyte/analysis Antigens, Protozoan/*immunology CD4-Positive T-Lymphocytes/immunology Cytotoxicity, Immunologic Immunity, Cellular Interferon Type II/physiology Interleukin-6/physiology Lymph Nodes/immunology Major Histocompatibility Complex Malaria/*immunology Mice Mice, Inbred Strains Peptides/immunology Plasmodium yoelii/*immunology *Protozoan Proteins T-Lymphocyte Subsets/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 15:26
Données d'usage