Modulation of activator protein-1 (AP-1) transcription factor and protein kinase C by hydrogen peroxide and D-alpha-tocopherol in vascular smooth muscle cells
Détails
ID Serval
serval:BIB_B811D6C9496E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Modulation of activator protein-1 (AP-1) transcription factor and protein kinase C by hydrogen peroxide and D-alpha-tocopherol in vascular smooth muscle cells
Périodique
European Journal of Biochemistry
ISSN
0014-2956 (Print)
Statut éditorial
Publié
Date de publication
12/1994
Volume
226
Numéro
2
Pages
393-402
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Résumé
The effects of hydrogen peroxide D-alpha-tocopherol and of D-beta-tocopherol on proliferation, protein kinase C and activator protein-1 (AP-1) activation have been studied in vascular smooth muscle cells. Cell proliferation, when activated by foetal calf serum, was inhibited by D-alpha-tocopherol. Protein kinase C activity was stimulated by hydrogen peroxide in a manner similar to phorbol myristate acetate; in the latter case, but not in the former, D-alpha-tocopherol inhibited the reaction. Hydrogen peroxide prevented phorbol-myristate-acetate-stimulated AP-1 binding to DNA but stimulated it if protein kinase C was down-regulated or inhibited. D-alpha-Tocopherol promoted AP-1 activation in quiescent cells but prevented its activation by phorbol myristate acetate. None of the described effects of D-alpha-tocopherol were shared by D-beta-tocopherol, suggesting a non-antioxidant mechanism as the basis of its action. The data show that hydrogen peroxide and D-alpha-tocopherol affect more than one element in the cell signal-transduction cascade.
Mots-clé
Animals
Aorta
Base Sequence
Blotting, Northern
Cell Division/drug effects
Cell Line
Cells, Cultured
DNA/metabolism
Gene Expression
Genes, fos
Genes, jun
Hydrogen Peroxide/*pharmacology
Molecular Sequence Data
Muscle, Smooth, Vascular/cytology/*drug effects/metabolism
Protein Kinase C/*metabolism
Rats
Tetradecanoylphorbol Acetate/pharmacology
Transcription Factor AP-1/*metabolism
Vitamin E/*pharmacology
Pubmed
Web of science
Création de la notice
29/01/2008 9:44
Dernière modification de la notice
20/08/2019 16:26