Tenascin-W, a new marker of cancer stroma, is elevated in sera of colon and breast cancer patients.

Détails

ID Serval
serval:BIB_B7E28FC01FAD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tenascin-W, a new marker of cancer stroma, is elevated in sera of colon and breast cancer patients.
Périodique
International Journal of Cancer = Journal international du cancer
Auteur(s)
Degen M., Brellier F., Schenk S., Driscoll R., Zaman K., Stupp R., Tornillo L., Terracciano L., Chiquet-Ehrismann R., Rüegg C., Seelentag W.
ISSN
1097-0215[electronic]
Statut éditorial
Publié
Date de publication
2008
Volume
122
Numéro
11
Pages
2454-2461
Langue
anglais
Résumé
Tenascins are extracellular matrix proteins present during the development of organisms as well as in pathological conditions. Tenascin-W, the fourth and last member of the tenascin family remains the least well-characterized one. Our study aimed to evaluate the potential significance of tenascin-W as cancer biomarker by monitoring its presence in the serum of colorectal and breast cancer patients and its expression in colorectal tumor tissues. To measure serum tenascin-W levels, a sensitive sandwich-ELISA was established. Mean tenascin-W concentration in sera of patients with nonmetastatic colorectal cancer at time of diagnosis was highly increased compared to that of healthy volunteers. A similar tendency was observed for tenascin-C in the same patient cohort. However, the increase was much more striking for tenascin-W. We also detected elevated tenascin-W levels in sera of breast cancer patients. Furthermore, we could show a prominent expression of tenascin-W in extracts from colorectal tumor tissues by immunoblot analysis, whereas tenascin-W was not detectable in the corresponding normal colon mucosa. To confirm the western blot results, we performed immunohistochemistry of frozen sections of the same patients as well as of an additional, independently chosen collection of colorectal cancer tissues. In all cases, similarly to tenascin-C, tenascin-W was detected in the tumor stroma. Our results reveal a clear association between elevated levels of tenascin-W and the presence of cancer. These results warrant further studies to evaluate the potential value of serum and tissue tenascin-W levels as diagnostic, prognostic or monitoring biomarker in colorectal, breast and possibly other solid cancers.
Mots-clé
Adult, Aged, Aged, 80 and over, Blotting, Western, Breast Neoplasms, Colorectal Neoplasms, Diagnosis, Differential, Enzyme-Linked Immunosorbent Assay, Female, Frozen Sections, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Intestinal Mucosa, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Predictive Value of Tests, Prognosis, Sensitivity and Specificity, Tenascin, Tumor Markers, Biological, Up-Regulation
Pubmed
Web of science
Création de la notice
17/06/2008 10:25
Dernière modification de la notice
20/08/2019 15:25
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