Inhibition of the lytic activity of perforin by lipoproteins
Détails
ID Serval
serval:BIB_B73C8F5DD558
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inhibition of the lytic activity of perforin by lipoproteins
Périodique
Journal of Immunology
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
09/1986
Volume
137
Numéro
6
Pages
1950-3
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 15
Research Support, Non-U.S. Gov't --- Old month value: Sep 15
Résumé
Cytoplasmic granules isolated from cytolytic T lymphocytes (CTL) lyse red blood cells or tumor cell lines in a nonspecific manner. The activity of highly purified granules was inhibited by human or rabbit serum at dilutions as high as 1/10,000. The main inhibitory activity of human serum was isolated by chromatography and was determined to be high density lipoprotein (HDL). HDL not only inhibited at a concentration of 70 ng/ml the lytic activity of isolated granules, but also of the purified, pore-forming protein perforin present in the granules. Purified low density lipoprotein was equally active. Because the CTL granule activity was inhibited by pure egg lecithin vesicles at a concentration equivalent to the phospholipid content of lipoproteins, the lipid portion of lipoproteins is the likely candidate for granule inactivation. Lipoproteins also decreased in a dose-dependent manner the cytotoxic activity of intact cytolytic T cells. However, cytotoxicity was not completely suppressed, and only in the case of CTL exhibiting low efficiency in killing their targets. It is proposed that lipoproteins inactivate perforin and may thereby inhibit a possible lysis of innocent bystander cells.
Mots-clé
Animals
Cell Survival/drug effects
Cytotoxicity, Immunologic/drug effects
Humans
Ion Channels
Lipoproteins, HDL/*pharmacology
*Membrane Glycoproteins
Membrane Proteins/*antagonists & inhibitors
Mice
Pore Forming Cytotoxic Proteins
T-Lymphocytes, Cytotoxic/drug effects/*physiology
Pubmed
Web of science
Création de la notice
24/01/2008 16:19
Dernière modification de la notice
20/08/2019 16:25