Disrupting the EMMPRIN (CD147)-cyclophilin A interaction reduces infarct size and preserves systolic function after myocardial ischemia and reperfusion.

Détails

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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_B69449C7BC88
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Disrupting the EMMPRIN (CD147)-cyclophilin A interaction reduces infarct size and preserves systolic function after myocardial ischemia and reperfusion.
Périodique
Arteriosclerosis, Thrombosis, and Vascular Biology
Auteur⸱e⸱s
Seizer P., Ochmann C., Schönberger T., Zach S., Rose M., Borst O., Klingel K., Kandolf R., MacDonald H.R., Nowak R.A., Engelhardt S., Lang F., Gawaz M., May A.E.
ISSN
1524-4636 (Electronic)
ISSN-L
1079-5642
Statut éditorial
Publié
Date de publication
2011
Volume
31
Numéro
6
Pages
1377-1386
Langue
anglais
Résumé
Objective-Inflammation and proteolysis crucially contribute to myocardial ischemia and reperfusion injury. The extracellular matrix metalloproteinase inducer EMMPRIN (CD147) and its ligand cyclophilin A (CyPA) may be involved in both processes. The aim of the study was to characterize the role of the CD147 and CyPA interplay in myocardial ischemia/reperfusion (I/R) injury.Methods and Results-Immunohistochemistry showed enhanced expression of CD147 and CyPA in myocardial sections from human autopsies of patients who had died from acute myocardial infarction and from mice at 24 hours after I/R. At 24 hours and 7 days after I/R, the infarct size was reduced in CD147(+/-) mice vs CD147(+/+) mice (C57Bl/6), in mice (C57Bl/6) treated with monoclonal antibody anti-CD147 vs control monoclonal antibody, and in CyPA(-/-) mice vs CyPA(+/+) mice (129S6/SvEv), all of which are associated with reduced monocyte and neutrophil recruitment at 24 hours and with a preserved systolic function at 7 days. The combination of CyPA(-/-) mice with anti-CD147 treatment did not yield further protection compared with either inhibition strategy alone. In vitro, treatment with CyPA induced monocyte chemotaxis in a CD147-and phosphatidylinositol 3-kinase-dependent manner and induced monocyte rolling and adhesion to endothelium (human umbilical vein endothelial cells) under flow in a CD147-dependent manner.Conclusion-CD147 and its ligand CyPA are inflammatory mediators after myocardial ischemia and reperfusion and represent potential targets to prevent myocardial I/R injury.
Mots-clé
Animals, Antigens, CD147/analysis, Antigens, CD147/physiology, Cell Adhesion, Cell Movement, Cyclophilin A/analysis, Cyclophilin A/physiology, Humans, Macrophages/physiology, Mice, Mice, Inbred C57BL, Myocardial Infarction/metabolism, Myocardial Ischemia/physiopathology, Myocardial Reperfusion Injury/etiology, Myocardial Reperfusion Injury/prevention & control, Neutrophils/physiology, Systole
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/02/2012 16:15
Dernière modification de la notice
20/08/2019 16:24
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