Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture.

Détails

ID Serval
serval:BIB_B67590F8BDC0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture.
Périodique
Brain Research
Auteur(s)
Honegger P., Richelson E.
ISSN
0006-8993 (Print)
ISSN-L
0006-8993
Statut éditorial
Publié
Date de publication
1976
Volume
109
Numéro
2
Pages
335-354
Langue
anglais
Résumé
Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic L-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.
Mots-clé
Acetylcholinesterase/metabolism, Animals, Animals, Newborn, Brain/embryology, Brain/enzymology, Carboxy-Lyases/metabolism, Catechol O-Methyltransferase/metabolism, Cell Aggregation, Cell Separation, Cells, Cultured, Choline O-Acetyltransferase/metabolism, Glutamate Decarboxylase/metabolism, Mice, Monoamine Oxidase/metabolism, Nerve Tissue Proteins/metabolism, Rats, Time Factors, Trypsin, Tyrosine 3-Monooxygenase/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 16:24
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