Involucrin mRNA is more abundant in human hair follicles than in normal epidermis

Détails

ID Serval
serval:BIB_B5A19C8A989F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Involucrin mRNA is more abundant in human hair follicles than in normal epidermis
Périodique
Journal of Investigative Dermatology
Auteur⸱e⸱s
de Viragh  P. A., Huber  M., Hohl  D.
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
12/1994
Volume
103
Numéro
6
Pages
815-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Involucrin is a precursor protein of the cornified cell envelope in epidermal keratinocytes, where it has been located by immunohistochemistry in the upper spinous and granular layers of human epidermis. In the hair follicle, involucrin has been found in the inner root sheath and in the upper layers of the infundibulum and the isthmus (upper outer root sheath), whereas its presence in the lower outer root sheath and the cortex has been controversial. Therefore, we analyzed the distribution of involucrin mRNA in adult scalp by Northern blotting and in situ hybridization. Northern blots showed more abundant involucrin mRNA in the follicular fraction than in the epidermal fraction of dissected scalp. In situ hybridization matched the immunohistologic results; transcripts of involucrin were expressed not only in the infundibulum and isthmus, but also in the hair cortex and medulla, in all layers of the inner root sheath, and in the inner cells of the lower outer root sheath (all of which lack a cell envelope at the ultrastructural level). However, involucrin was absent in the hair cuticle, which is the only compartment of the follicle possessing a morphologically distinct cell envelope. Our results suggest, first, that involucrin does not serve as a precursor protein of the cornified cell envelope in adult hair follicles, and second, that it is perhaps not necessary for the formation of the cell envelope in keratinocytes of the hair cuticle, as we did not find this precursor protein with highly sensitive methodology.
Mots-clé
Blotting, Northern Hair/*chemistry Humans Immunohistochemistry In Situ Hybridization Protein Precursors/*genetics RNA, Messenger/analysis Skin/*chemistry
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:35
Dernière modification de la notice
20/08/2019 15:24
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