CD99 is upregulated in placenta and astrocytomas with a differential subcellular distribution according to the malignancy stage.

Détails

ID Serval
serval:BIB_B595A19E5830
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CD99 is upregulated in placenta and astrocytomas with a differential subcellular distribution according to the malignancy stage.
Périodique
Journal of Neuro-oncology
Auteur(s)
Urias U., Marie S.K., Uno M., da Silva R., Evagelinellis M.M., Caballero O.L., Stevenson B.J., Silva W.A., Simpson A.J., Oba-Shinjo S.M.
ISSN
1573-7373 (Electronic)
ISSN-L
0167-594X
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
119
Numéro
1
Pages
59-70
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
In the present study, we searched for genes highly expressed in placenta and that could contribute to the establishment and maintenance of a malignant phenotype in different types of tumours, and in astrocytomas in particular. We employed a strategy based on the integration of in silico data from previously generated massively parallel signature sequencing and public serial analysis of gene expression databases. Among 12 selected genes, CD99 exhibited the highest relative mRNA expression in GBM compared to non-neoplastic brain tissues. In a larger cohort of astrocytic tumours, we further demonstrated increased CD99 expression in all malignant grades, with GBMs showing the highest values. These findings were confirmed at the protein level by Western blotting and immunohistochemistry. Additionally, we demonstrated the CD99 localisation profile in astrocytic tumours. Interestingly, CD99 expression was confined to the cytoplasm or membrane in more malignant astrocytomas, in contrast to non-neoplastic brain tissue or non-infiltrative pilocytic astrocytoma, which showed no obvious staining in these structures. Comparison of three GBM cell lines revealed higher CD99 expression at the membrane and higher migratory capacity in the A172 and U87MG lines, but lower CD99 expression and no migratory ability in the T98 line. Knocking down CD99 expression by siRNA decreased significantly the migration of both cell lines. These integrated CD99 gene and protein expression results suggest that CD99 expression in astrocytomas of different malignant grades might contribute to the infiltrative ability and support the importance of CD99 as a potential target to reduce infiltrative astrocytoma capacity in migration and invasion.
Pubmed
Web of science
Création de la notice
19/09/2014 18:56
Dernière modification de la notice
20/08/2019 16:24
Données d'usage