Measurement of immunoreactive angiotensin-(1-7) heptapeptide in human blood.

Détails

ID Serval
serval:BIB_B58CFB2CE5A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Measurement of immunoreactive angiotensin-(1-7) heptapeptide in human blood.
Périodique
Clinical chemistry
Auteur⸱e⸱s
Nussberger J., Brunner D.B., Nyfeler J.A., Linder L., Brunner H.R.
ISSN
0009-9147
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
47
Numéro
4
Pages
726-9
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
BACKGROUND: The renal enzyme renin cleaves from the hepatic alpha(2)-globulin angiotensinogen angiotensin-(1-10) decapeptide [Ang-(1-10)], which is further metabolized to smaller peptides that help maintain cardiovascular homeostasis. The Ang-(1-7) heptapeptide has been reported to have several physiological effects, including natriuresis, diuresis, vasodilation, and release of vasopressin and prostaglandins. METHODS: To investigate Ang-(1-7) in clinical settings, we developed a method to measure immunoreactive (ir-) Ang-(1-7) in 2 mL of human blood and to estimate plasma concentrations by correcting for the hematocrit. A sensitive and specific antiserum against Ang-(1-7) was raised in a rabbit. Human blood was collected in the presence of an inhibitor mixture including a renin inhibitor to prevent peptide generation in vitro. Ang-(1-7) was extracted into ethanol and purified on phenylsilylsilica. The peptide was quantified by radioimmunoassay. Increasing doses of Ang-(1-7) were infused into volunteers, and plasma concentrations of the peptide were measured. RESULTS: The detection limit for plasma ir-Ang-(1-7) was 1 pmol/L. CVs for high and low blood concentrations were 4% and 20%, respectively, and between-assay CVs were 8% and 13%, respectively. Reference values for human plasma concentrations of ir-Ang-(1-7) were 1.0-9.5 pmol/L (median, 4.7 pmol/L) and increased linearly during infusion of increasing doses of Ang-(1-7). CONCLUSIONS: Reliable measurement of plasma ir-Ang-(1-7) is achieved with efficient inhibition of enzymes that generate or metabolize Ang-(1-7) after blood sampling, extraction in ethanol, and purification on phenylsilylsilica, and by use of a specific antiserum.
Mots-clé
Adult, Angiotensin I, Humans, Middle Aged, Peptide Fragments, Radioimmunoassay, Reference Values, Reproducibility of Results
Pubmed
Web of science
Création de la notice
05/03/2008 16:40
Dernière modification de la notice
20/08/2019 15:24
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