Emerging single-cell technologies in immunology.
Détails
ID Serval
serval:BIB_B577FB613E4C
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Emerging single-cell technologies in immunology.
Périodique
Journal of Leukocyte Biology
ISSN
1938-3673 (Electronic)
ISSN-L
0741-5400
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
98
Numéro
1
Pages
23-32
Langue
anglais
Notes
Publication types: Journal Article ; Review Publication Status: ppublish Document Type: Review
Résumé
During evolution, the immune system has diversified to protect the host from the extremely wide array of possible pathogens. Until recently, immune responses were dissected by use of global approaches and bulk tools, averaging responses across samples and potentially missing particular contributions of individual cells. This is a strongly limiting factor, considering that initial immune responses are likely to be triggered by a restricted number of cells at the vanguard of host defenses. The development of novel, single-cell technologies is a major innovation offering great promise for basic and translational immunology with the potential to overcome some of the limitations of traditional research tools, such as polychromatic flow cytometry or microscopy-based methods. At the transcriptional level, much progress has been made in the fields of microfluidics and single-cell RNA sequencing. At the protein level, mass cytometry already allows the analysis of twice as many parameters as flow cytometry. In this review, we explore the basis and outcome of immune-cell diversity, how genetically identical cells become functionally different, and the consequences for the exploration of host-immune defense responses. We will highlight the advantages, trade-offs, and potential pitfalls of emerging, single-cell-based technologies and how they provide unprecedented detail of immune responses.
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/07/2015 13:47
Dernière modification de la notice
27/08/2024 10:21