Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface.

Détails

ID Serval
serval:BIB_B51D8D87BEB7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface.
Périodique
Nature
Auteur(s)
Dale G.L., Friese P., Batar P., Hamilton S.F., Reed G.L., Jackson K.W., Clemetson K.J., Alberio L.
ISSN
0028-0836 (Print)
ISSN-L
0028-0836
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
415
Numéro
6868
Pages
175-179
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Activated platelets bind numerous adhesive and procoagulant proteins by receptor-mediated processes. Although there is little evidence to suggest that these processes are heterogeneous in platelets, we previously found that platelets co-stimulated with collagen and thrombin express functional alpha-granule factor V only on a subpopulation of cells. Here we show that these cells, referred to as 'COAT-platelets', bind additional alpha-granule proteins, including fibrinogen, von Willebrand factor, thrombospondin, fibronectin and alpha2-antiplasmin. These proteins are all transglutaminase substrates, and inhibitors of transglutaminase prevent the production of COAT-platelets. A synthetic transglutaminase substrate (CP15) also binds to COAT-platelets, and analysis by high performance liquid chromatography/mass spectrometry shows that a product is formed with a relative molecular mass (Mr) equal to CP15 plus 176. Serotonin, an abundant component of platelet-dense granules, has an Mr of 176, and fibrinogen isolated from COAT-platelets contains covalently linked serotonin. Synthetic bovine serum albumin-(serotonin)6 binds selectively to COAT-platelets and also inhibits the retention of procoagulant proteins on COAT-platelets. These data indicate that COAT-platelets use serotonin conjugation to augment the retention of procoagulant proteins on their cell surface through an as yet unidentified serotonin receptor.
Mots-clé
Amino Acid Sequence, Animals, Biotin/metabolism, Blood Coagulation, Blood Coagulation Factors, Blood Platelets/metabolism, Cell Membrane/metabolism, Factor V/metabolism, Factor XIII/metabolism, Fibrinogen, Humans, Molecular Sequence Data, Peptides/metabolism, Platelet Activation, Platelet Glycoprotein GPIb-IX Complex/metabolism, Protein Binding, Serotonin, Transglutaminases/metabolism
Pubmed
Web of science
Création de la notice
10/02/2015 11:05
Dernière modification de la notice
20/08/2019 15:23
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