Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells

Détails

ID Serval
serval:BIB_B4E597BB1A91
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells
Périodique
Experimental Cell Research
Auteur(s)
Kataoka  T., Ito  M., Budd  R. C., Tschopp  J., Nagai  K.
ISSN
0014-4827 (Print)
Statut éditorial
Publié
Date de publication
02/2002
Volume
273
Numéro
2
Pages
256-64
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb 15
Résumé
The caspase-8 inhibitor c-FLIP blocks death receptor-mediated cell death and plays an essential role in the regulation of lymphocyte homeostasis and the immune escape of tumors. The murine thymoma cell line EL-4 was resistant to Fas ligand (FasL)-induced apoptosis by constitutive expression of FLIP (L). Cycloheximide downregulated the expression of FLIP (L) and markedly sensitized EL-4 cells to FasL-induced apoptosis. In contrast, DNA-damaging agents sensitized EL-4 cells to FasL-induced cell death via an increase of cell-surface Fas without any influence on FLIP (L) expression. Enforced expression of transfected Fas rendered EL-4 cells highly susceptible to FasL-induced cell death. These findings demonstrate that susceptibility to FasL-induced cell death mainly depends on the expression level of c-FLIP versus cell-surface Fas.
Mots-clé
Animals Antigens, CD95/*metabolism *Apoptosis CASP8 and FADD-Like Apoptosis Regulating Protein Camptothecin/pharmacology Carrier Proteins/*genetics Caspases/*antagonists & inhibitors Cell Membrane/metabolism Cycloheximide/pharmacology Down-Regulation Doxorubicin/pharmacology Etoposide/pharmacology Fas Ligand Protein *Gene Expression *Intracellular Signaling Peptides and Proteins Membrane Glycoproteins/*metabolism Mice Mitomycin/pharmacology Protein Synthesis Inhibitors/pharmacology Thymoma Trans-Activation (Genetics) Transfection Tumor Cells, Cultured Up-Regulation
Pubmed
Web of science
Création de la notice
24/01/2008 15:18
Dernière modification de la notice
20/08/2019 15:23
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