Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells
Détails
ID Serval
serval:BIB_B4E597BB1A91
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells
Périodique
Experimental Cell Research
ISSN
0014-4827 (Print)
Statut éditorial
Publié
Date de publication
02/2002
Volume
273
Numéro
2
Pages
256-64
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb 15
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb 15
Résumé
The caspase-8 inhibitor c-FLIP blocks death receptor-mediated cell death and plays an essential role in the regulation of lymphocyte homeostasis and the immune escape of tumors. The murine thymoma cell line EL-4 was resistant to Fas ligand (FasL)-induced apoptosis by constitutive expression of FLIP (L). Cycloheximide downregulated the expression of FLIP (L) and markedly sensitized EL-4 cells to FasL-induced apoptosis. In contrast, DNA-damaging agents sensitized EL-4 cells to FasL-induced cell death via an increase of cell-surface Fas without any influence on FLIP (L) expression. Enforced expression of transfected Fas rendered EL-4 cells highly susceptible to FasL-induced cell death. These findings demonstrate that susceptibility to FasL-induced cell death mainly depends on the expression level of c-FLIP versus cell-surface Fas.
Mots-clé
Animals
Antigens, CD95/*metabolism
*Apoptosis
CASP8 and FADD-Like Apoptosis Regulating Protein
Camptothecin/pharmacology
Carrier Proteins/*genetics
Caspases/*antagonists & inhibitors
Cell Membrane/metabolism
Cycloheximide/pharmacology
Down-Regulation
Doxorubicin/pharmacology
Etoposide/pharmacology
Fas Ligand Protein
*Gene Expression
*Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins/*metabolism
Mice
Mitomycin/pharmacology
Protein Synthesis Inhibitors/pharmacology
Thymoma
Trans-Activation (Genetics)
Transfection
Tumor Cells, Cultured
Up-Regulation
Pubmed
Web of science
Création de la notice
24/01/2008 16:18
Dernière modification de la notice
20/08/2019 16:23