Interactions of Ly49 family receptors with MHC class I ligands in trans and cis.

Détails

ID Serval
serval:BIB_B4CA2A35F031
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interactions of Ly49 family receptors with MHC class I ligands in trans and cis.
Périodique
Journal of immunology
Auteur(s)
Scarpellino L., Oeschger F., Guillaume P., Coudert J.D., Lévy F., Leclercq G., Held W.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
178
Numéro
3
Pages
1277-1284
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The Ly49A NK cell receptor interacts with MHC class I (MHC-I) molecules on target cells and negatively regulates NK cell-mediated target cell lysis. We have recently shown that the MHC-I ligand-binding capacity of the Ly49A NK cell receptor is controlled by the NK cells' own MHC-I. To see whether this property was unique to Ly49A, we have investigated the binding of soluble MHC-I multimers to the Ly49 family receptors expressed in MHC-I-deficient and -sufficient C57BL/6 mice. In this study, we confirm the binding of classical MHC-I to the inhibitory Ly49A, C and I receptors, and demonstrate that detectable MHC-I binding to MHC-I-deficient NK cells is exclusively mediated by these three receptors. We did not detect significant multimer binding to stably transfected or NK cell-expressed Ly49D, E, F, G, and H receptors. Yet, we identified the more distantly related Ly49B and Ly49Q, which are not expressed by NK cells, as two novel MHC-I receptors in mice. Furthermore, we show using MHC-I-sufficient mice that the NK cells' own MHC-I significantly masks the Ly49A and Ly49C, but not the Ly49I receptor. Nevertheless, Ly49I was partly masked on transfected tumor cells, suggesting that the structure of Ly49I is compatible in principal with cis binding of MHC-I. Finally, masking of Ly49Q by cis MHC-I was minor, whereas masking of Ly49B was not detected. These data significantly extend the MHC-I specificity of Ly49 family receptors and show that the accessibility of most, but not all, MHC-I-binding Ly49 receptors is modulated by the expression of MHC-I in cis.
Mots-clé
Animals, Antigens, Ly/metabolism, Histocompatibility Antigens Class I/metabolism, Killer Cells, Natural/immunology, Lectins, C-Type/metabolism, Ligands, Mice, Mice, Inbred C57BL, NK Cell Lectin-Like Receptor Subfamily A, Protein Binding, Receptors, Immunologic/metabolism, Receptors, NK Cell Lectin-Like
Pubmed
Web of science
Création de la notice
28/01/2008 11:17
Dernière modification de la notice
20/08/2019 15:23
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