Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_B4AA7325E6FA
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.
Périodique
Autophagy
Auteur(s)
Klionsky D.J., Abeliovich H., Agostinis P., Agrawal D.K., Aliev G., Askew D.S., Baba M., Baehrecke E.H., Bahr B.A., Ballabio A., Bamber B.A., Bassham D.C., Bergamini E., Bi X., Biard-Piechaczyk M., Blum J.S., Bredesen D.E., Brodsky J.L., Brumell J.H., Brunk U.T., Bursch W., Camougrand N., Cebollero E., Cecconi F., Chen Y., Chin L.S., Choi A., Chu C.T., Chung J., Clarke P.G.H, et al.
ISSN
1554-8635
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
4
Numéro
2
Pages
151-175
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Review
Résumé
Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
Mots-clé
Animals, Autophagy/physiology, Data Interpretation, Statistical, Eukaryotic Cells/physiology, Guidelines as Topic, Humans, Laboratory Techniques and Procedures, Microscopy, Fluorescence/methods, Microtubule-Associated Proteins/metabolism, Models, Biological, Phagosomes/metabolism, Phagosomes/physiology, Plants/metabolism, Protein Processing, Post-Translational, Protein Transport, Saccharomyces cerevisiae Proteins/metabolism
Pubmed
Web of science
Création de la notice
17/03/2009 10:51
Dernière modification de la notice
20/08/2019 15:23
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