Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis

Détails

ID Serval
serval:BIB_B49C4AA15051
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis
Périodique
Journal of Immunology
Auteur⸱e⸱s
Luther  S. A., Bidgol  A., Hargreaves  D. C., Schmidt  A., Xu  Y., Paniyadi  J., Matloubian  M., Cyster  J. G.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
07/2002
Volume
169
Numéro
1
Pages
424-33
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 1
Résumé
Despite their widespread expression, the in vivo recruitment activities of CCL19 (EBV-induced molecule 1 ligand chemokine) and CXCL12 (stromal cell-derived factor 1) have not been established. Furthermore, although CXCL13 (B lymphocyte chemoattractant) has been shown to induce lymphoid neogenesis through induction of lymphotoxin (LT)alpha1beta2, it is unclear whether other homeostatic chemokines have this property. In this work we show that ectopic expression in pancreatic islets of CCL19 leads to small infiltrates composed of lymphocytes and dendritic cells and containing high endothelial venules and stromal cells. Ectopic CXCL12 induced small infiltrates containing few T cells but enriched in dendritic cells, B cells, and plasma cells. Comparison of CCL19 transgenic mice with mice expressing CCL21 (secondary lymphoid tissue chemokine) revealed that CCL21 induced larger and more organized infiltrates. A more significant role for CCL21 is also suggested in lymphoid tissues, as CCL21 protein was found to be present in lymph nodes and spleen at much higher concentrations than CCL19. CCL19 and CCL21 but not CXCL12 induced LTalpha1beta2 expression on naive CD4 T cells, and treatment of CCL21 transgenic mice with LTbetaR-Fc antagonized development of organized lymphoid structures. LTalpha1beta2 was also induced on naive T cells by the cytokines IL-4 and IL-7. These studies establish that CCL19 and CXCL12 are sufficient to mediate cell recruitment in vivo and they indicate that LTalpha1beta2 may function downstream of CCL21, CCL19, and IL-2 family cytokines in normal and pathological lymphoid tissue development.
Mots-clé
Animals Cell Differentiation/immunology Cell Movement/*immunology Chemokines, CC/biosynthesis/genetics/*physiology Chemokines, CXC/biosynthesis/*physiology Cytokines/pharmacology Dendritic Cells/*cytology/immunology Endothelium, Lymphatic/immunology/pathology Female Homeostasis/*immunology Immunoglobulin Fc Fragments/pharmacology Islets of Langerhans/immunology/metabolism Lymphocyte Subsets/*cytology/immunology Lymphoid Tissue/*cytology/immunology Lymphotoxin beta Receptor Lymphotoxin-alpha/biosynthesis Lymphotoxin-beta Membrane Proteins/biosynthesis Mice Mice, Inbred C57BL Mice, Inbred NOD Mice, Transgenic Nuclear Proteins/genetics Plasma Cells/cytology/immunology Rats Receptors, Tumor Necrosis Factor/physiology Stromal Cells/pathology T-Lymphocytes/immunology/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:04
Dernière modification de la notice
20/08/2019 15:23
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