Plasminogen activators in dextran sulfate-activated euglobulin fractions: a molecular analysis of factor XII- and prekallikrein-dependent fibrinolysis

Détails

ID Serval
serval:BIB_B47D6A1FFAFA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasminogen activators in dextran sulfate-activated euglobulin fractions: a molecular analysis of factor XII- and prekallikrein-dependent fibrinolysis
Périodique
Blood
Auteur(s)
Hauert  J., Nicoloso  G., Schleuning  W. D., Bachmann  F., Schapira  M.
ISSN
0006-4971 (Print)
Statut éditorial
Publié
Date de publication
03/1989
Volume
73
Numéro
4
Pages
994-9
Notes
Journal Article --- Old month value: Mar
Résumé
To elucidate the mechanism by which activation of the contact system of blood coagulation leads to expression of fibrinolytic activity, we have determined the molecular characteristics of the plasminogen activators present in dextran sulfate-treated euglobulin fractions by electrophoretic-zymographic analysis and specific immunoadsorption. In addition to free and protease inhibitor-bound tissue-type plasminogen activator (t-PA), dextran sulfate precipitates of euglobulins contained the complex formed between plasma kallikrein and C1-inhibitor, an indicator of prekallikrein activation. These precipitates also contained substantial fibrinolytic activity related to urinary-type plasminogen activator (u-PA). Autoradiographic analysis was then used to evaluate the cleavage of 125I-single-chain u-PA (prourokinase) in dextran sulfate euglobulins as well as after exposure to kallikrein or beta-factor XIIa. This analysis supported the conclusion that plasma kallikrein-mediated cleavage and activation of single-chain u-PA is the mechanism operative for the development of lytic activity in euglobulin precipitates following activation of the contact system.
Mots-clé
Blood Proteins/metabolism Dextran Sulfate Dextrans/*metabolism Electrophoresis Factor XII/metabolism *Fibrinolysis Humans Plasminogen Activators/*metabolism Prekallikrein/metabolism Serum Globulins/*metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 15:28
Dernière modification de la notice
20/08/2019 15:22
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