Closing the gap between T-cell life span estimates from stable isotope-labeling studies in mice and humans.

Détails

ID Serval
serval:BIB_B3B058597732
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Closing the gap between T-cell life span estimates from stable isotope-labeling studies in mice and humans.
Périodique
Blood
Auteur⸱e⸱s
Westera L., Drylewicz J., den Braber I., Mugwagwa T., van der Maas I., Kwast L., Volman T., van de Weg-Schrijver E.H., Bartha I., Spierenburg G., Gaiser K., Ackermans M.T., Asquith B., de Boer R.J., Tesselaar K., Borghans J.A.
ISSN
1528-0020 (Electronic)
ISSN-L
0006-4971
Statut éditorial
Publié
Date de publication
2013
Volume
122
Numéro
13
Pages
2205-2212
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Quantitative knowledge of the turnover of different leukocyte populations is a key to our understanding of immune function in health and disease. Much progress has been made thanks to the introduction of stable isotope labeling, the state-of-the-art technique for in vivo quantification of cellular life spans. Yet, even leukocyte life span estimates on the basis of stable isotope labeling can vary up to 10-fold among laboratories. We investigated whether these differences could be the result of variances in the length of the labeling period among studies. To this end, we performed deuterated water-labeling experiments in mice, in which only the length of label administration was varied. The resulting life span estimates were indeed dependent on the length of the labeling period when the data were analyzed using a commonly used single-exponential model. We show that multiexponential models provide the necessary tool to obtain life span estimates that are independent of the length of the labeling period. Use of a multiexponential model enabled us to reduce the gap between human T-cell life span estimates from 2 previously published labeling studies. This provides an important step toward unambiguous understanding of leukocyte turnover in health and disease.
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/11/2013 20:58
Dernière modification de la notice
20/08/2019 15:22
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