Acetylcholine receptors in human thymic myoid cells in situ: an immunohistological study

Détails

ID Serval
serval:BIB_B2D621EB83EF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Acetylcholine receptors in human thymic myoid cells in situ: an immunohistological study
Périodique
Annals of Neurology
Auteur(s)
Schluep  M., Willcox  N., Vincent  A., Dhoot  G. K., Newsom-Davis  J.
ISSN
0364-5134 (Print)
Statut éditorial
Publié
Date de publication
08/1987
Volume
22
Numéro
2
Pages
212-22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
Myoid cells were studied by double immunofluorescence in sections of thymus from 47 patients with myasthenia gravis and 15 control subjects, using polyclonal sheep anti-troponin T and monoclonal antibodies to troponin I, striated muscle myosin, and acetylcholine receptor (AChR). The myoid cells were rare and located mainly in the medulla, and most were clearly positive for AChR; labeling was similar with four individual monoclonal antibodies specific for extrajunctional AChR and five that also recognize endplate AChR. They were mostly keratin-positive and consistently HLA-DR-negative. In the myasthenia gravis samples, the myoid cells were similar but largely confined to medullary epithelial areas; AChR labeling was slightly weaker, but otherwise they did not differ noticeably from those of control subjects. A preliminary finding was of even rarer AChR-positive/HLA-DR-positive antigen-presenting (possibly) cells seen in 9 of 9 myasthenia gravis samples and in none of 9 control samples. Although myoid-cell AChR appears antigenically similar to extrajunctional muscle AChR, and must therefore express the epitopes that myasthenics' antibodies recognize, these cells do not appear to be foci of immunological stimulation in myasthenia gravis.
Mots-clé
Binding Sites Histocytochemistry Humans Immunochemistry Muscles/*metabolism/pathology Myasthenia Gravis/metabolism/pathology Receptors, Cholinergic/*metabolism Reference Values Thymus Gland/*metabolism/pathology
Pubmed
Web of science
Création de la notice
25/01/2008 12:46
Dernière modification de la notice
20/08/2019 15:21
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