Variability between laboratories performing coagulation tests with identical platforms: a nationwide evaluation study.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_B2A91169F7A2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Variability between laboratories performing coagulation tests with identical platforms: a nationwide evaluation study.
Périodique
Thrombosis Journal
Auteur(s)
Nagler M., Bachmann L.M., Alberio L., Angelillo-Scherrer A., Asmis L.M., Korte W., Mendez A., Reber G., Stricker H., Tsakiris D.A., Wuillemin W.A.
ISSN
1477-9560 (Electronic)
ISSN-L
1477-9560
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
11
Numéro
1
Pages
6
Langue
anglais
Notes
Publication types: Journal Article Publication Status: epublish
Résumé
BACKGROUND: While the assessment of analytical precision within medical laboratories has received much attention in scientific enquiry, the degree of as well as the sources causing variation between them remains incompletely understood. In this study, we quantified the variance components when performing coagulation tests with identical analytical platforms in different laboratories and computed intraclass correlations coefficients (ICC) for each coagulation test.
METHODS: Data from eight laboratories measuring fibrinogen twice in twenty healthy subjects with one out of 3 different platforms and single measurements of prothrombin time (PT), and coagulation factors II, V, VII, VIII, IX, X, XI and XIII were analysed. By platform, the variance components of (i) the subjects, (ii) the laboratory and the technician and (iii) the total variance were obtained for fibrinogen as well as (i) and (iii) for the remaining factors using ANOVA.
RESULTS: The variability for fibrinogen measurements within a laboratory ranged from 0.02 to 0.04, the variability between laboratories ranged from 0.006 to 0.097. The ICC for fibrinogen ranged from 0.37 to 0.66 and from 0.19 to 0.80 for PT between the platforms. For the remaining factors the ICC's ranged from 0.04 (FII) to 0.93 (FVIII).
CONCLUSIONS: Variance components that could be attributed to technicians or laboratory procedures were substantial, led to disappointingly low intraclass correlation coefficients for several factors and were pronounced for some of the platforms. Our findings call for sustained efforts to raise the level of standardization of structures and procedures involved in the quantification of coagulation factors.
Pubmed
Open Access
Oui
Création de la notice
10/02/2015 9:43
Dernière modification de la notice
20/08/2019 15:21
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