Combined structural and chemical analysis of the anammoxosome: a membrane-bounded intracytoplasmic compartment in anammox bacteria.

Détails

ID Serval
serval:BIB_B299E74B759D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Combined structural and chemical analysis of the anammoxosome: a membrane-bounded intracytoplasmic compartment in anammox bacteria.
Périodique
Journal of Structural Biology
Auteur⸱e⸱s
van Niftrik L., Geerts W.J., van Donselaar E.G., Humbel B.M., Yakushevska A., Verkleij A.J., Jetten M.S., Strous M.
ISSN
1095-8657 (Electronic)
ISSN-L
1047-8477
Statut éditorial
Publié
Date de publication
2008
Volume
161
Numéro
3
Pages
401-410
Langue
anglais
Résumé
Anammox bacteria have unique intracellular membranes that divide their cytoplasm into three separate compartments. The largest and innermost cytoplasmic compartment, the anammoxosome, is hypothesized to be the locus of all catabolic reactions in the anammox metabolism. Electron tomography showed that the anammoxosome and its membrane were highly folded. This finding was confirmed by a transmission electron microscopy study using different sample preparation methods. Further, in this study electron-dense particles were observed and electron tomography showed that they were confined to the anammoxosome compartment. Energy dispersive X-ray analysis revealed that these particles contained iron. The functional significance of a highly folded anammoxosome membrane and intracellular iron storage particles are discussed in relation to their possible function in energy generation.
Mots-clé
Bacteria/chemistry, Cell Compartmentation, Cryopreservation, Cryoultramicrotomy, Cytoplasm/ultrastructure, Lipids/analysis, Microscopy, Electron, Transmission, Species Specificity, Tomography
Pubmed
Web of science
Création de la notice
28/02/2012 18:44
Dernière modification de la notice
20/08/2019 15:21
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