The Release Kinetics of Eosinophil Peroxidase and Mitochondrial DNA Is Different in Association with Eosinophil Extracellular Trap Formation.

Détails

Ressource 1Télécharger: 33546138_BIB_B2860403C015.pdf (2648.13 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_B2860403C015
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The Release Kinetics of Eosinophil Peroxidase and Mitochondrial DNA Is Different in Association with Eosinophil Extracellular Trap Formation.
Périodique
Cells
Auteur⸱e⸱s
Germic N., Fettrelet T., Stojkov D., Hosseini A., Horn M.P., Karaulov A., Simon D., Yousefi S., Simon H.U.
ISSN
2073-4409 (Electronic)
ISSN-L
2073-4409
Statut éditorial
Publié
Date de publication
03/02/2021
Peer-reviewed
Oui
Volume
10
Numéro
2
Pages
306
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Eosinophils are a subset of granulocytes characterized by a high abundance of specific granules in their cytoplasm. To act as effector cells, eosinophils degranulate and form eosinophil extracellular traps (EETs), which contain double-stranded DNA (dsDNA) co-localized with granule proteins. The exact molecular mechanism of EET formation remains unknown. Although the term "EET release" has been used in scientific reports, it is unclear whether EETs are pre-formed in eosinophils and subsequently released. Moreover, although eosinophil degranulation has been extensively studied, a precise time-course of granule protein release has not been reported until now. In this study, we investigated the time-dependent release of eosinophil peroxidase (EPX) and mitochondrial DNA (mtDNA) following activation of both human and mouse eosinophils. Unexpectedly, maximal degranulation was already observed within 1 min with no further change upon complement factor 5 (C5a) stimulation of interleukin-5 (IL-5) or granulocyte/macrophage colony-stimulating factor (GM-CSF)-primed eosinophils. In contrast, bulk mtDNA release in the same eosinophil populations occurred much slower and reached maximal levels between 30 and 60 min. Although no single-cell analyses have been performed, these data suggest that the molecular pathways leading to degranulation and mtDNA release are at least partially different. Moreover, based on these data, it is likely that the association between the mtDNA scaffold and granule proteins in the process of EET formation occurs in the extracellular space.
Mots-clé
degranulation, eosinophil extracellular traps, eosinophil peroxidase, eosinophils, kinetics, mitochondrial DNA
Pubmed
Web of science
Open Access
Oui
APC
1600 CHF
Création de la notice
22/02/2021 15:52
Dernière modification de la notice
30/04/2021 7:14
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