In vivo gene delivery of HSP70i by adenovirus and adeno-associated virus preserves contractile function in mouse heart following ischemia-reperfusion.

Détails

ID Serval
serval:BIB_B260FBE3D15B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo gene delivery of HSP70i by adenovirus and adeno-associated virus preserves contractile function in mouse heart following ischemia-reperfusion.
Périodique
American Journal of Physiology. Heart and Circulatory Physiology
Auteur(s)
Belke D.D., Gloss B., Hollander J.M., Swanson E.A., Duplain H., Dillmann W.H.
ISSN
0363-6135
Statut éditorial
Publié
Date de publication
12/2006
Peer-reviewed
Oui
Volume
291
Numéro
6
Pages
H2905-2910
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Inducible heat shock protein 70 (HSP70i) has been shown to exert a protective effect in hearts subjected to ischemia-reperfusion. Although studied in heat-shocked animals and in transgenic mice that constitutively overexpress the protein, the therapeutic application of the protein in the form of a viral vector-mediated HSP70i expression has not been widely examined. Accordingly, we have examined the effects of HSP70i delivered in vivo to the left ventricular free wall of the heart via viral gene therapy in mice. The affect of virally mediated HSP70i expression in preserving cardiac function following ischemia-reperfusion was examined after short-term expression (5-day adenovirus mediated) and long-term expression (8-mo adeno-associated virus mediated) in mice by subjecting ex vivo Langendorff perfused hearts to a regime of ischemia-reperfusion. Both vectors were capable of increasing HSP70i expression in the heart, and neither vector had any effect on cardiac function during aerobic (preischemic) perfusion when compared with corresponding controls. In contrast, both adenovirus-mediated and adeno-associated virus-mediated expression of HSP70i improved the contractile recovery of the heart after 120 min of reperfusion following ischemia. This study demonstrates the feasibility of using both short- and long-term expression of virally mediated HSP70i as a therapeutic intervention against cardiac ischemia-reperfusion injury.
Mots-clé
Adenoviridae/genetics, Adenoviridae/metabolism, Animals, Dependovirus/genetics, Dependovirus/metabolism, Gene Expression Regulation/physiology, Gene Therapy/methods, Genetic Vectors, HSP70 Heat-Shock Proteins/genetics, HSP70 Heat-Shock Proteins/metabolism, Heart Ventricles/virology, Lac Operon/genetics, Lac Operon/physiology, Mice, Myocardial Contraction/genetics, Myocardial Contraction/physiology, Myocardial Reperfusion Injury/genetics, Myocardial Reperfusion Injury/metabolism, Ventricular Function
Pubmed
Web of science
Création de la notice
25/01/2008 13:44
Dernière modification de la notice
20/08/2019 15:21
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